TY - JOUR
T1 - Characterization of a microbacterium sp. Strain isolated from soils contaminated with hydrocarbons in the Burgos Basin, Mexico
AU - Cruz-Hernández, María Antonia
AU - Reyes-Peralta, Jessica
AU - Mendoza-Herrera, Alberto
AU - Rivera, Gildardo
AU - Bocanegra-García, Virgilio
N1 - Publisher Copyright:
© 2021, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved.
PY - 2021
Y1 - 2021
N2 - The development of novel bioremediation strategies has focused on the isolation and identification of microorganisms that can thrive in polluted environments to evaluate their potential as biotechnological tools in bioremediation techniques. In this work, a bacterium isolated from hydrocarbon-contaminated soils from the Burgos basin was identified and its hydrocarbon degradation potential was evaluated. Identification based on sequencing the 16S rRNA gene identified one of the isolates (R3) as Microbacterium petrolearium. This strain was mainly antibiotic-sensitive with elevated carbohydrate assimilation differing from previously reported strains. Moderate surfactant production (I24 = 22.97 %) was observed, which was absent in a cell-free extract. M. petrolearium R3 showed increased growth that correlated with pollutant concentration. For light crude oil, at a higher contaminant percentage, the R3 strain showed increased growth; however, in the case of diesel, no growth was detected. The aforementioned data indicate that M. petrolearium strain R3, isolated from local sources, has potential use as a tool for hydrocarbon-contaminated soil bioremediation.
AB - The development of novel bioremediation strategies has focused on the isolation and identification of microorganisms that can thrive in polluted environments to evaluate their potential as biotechnological tools in bioremediation techniques. In this work, a bacterium isolated from hydrocarbon-contaminated soils from the Burgos basin was identified and its hydrocarbon degradation potential was evaluated. Identification based on sequencing the 16S rRNA gene identified one of the isolates (R3) as Microbacterium petrolearium. This strain was mainly antibiotic-sensitive with elevated carbohydrate assimilation differing from previously reported strains. Moderate surfactant production (I24 = 22.97 %) was observed, which was absent in a cell-free extract. M. petrolearium R3 showed increased growth that correlated with pollutant concentration. For light crude oil, at a higher contaminant percentage, the R3 strain showed increased growth; however, in the case of diesel, no growth was detected. The aforementioned data indicate that M. petrolearium strain R3, isolated from local sources, has potential use as a tool for hydrocarbon-contaminated soil bioremediation.
KW - Bioremediation
KW - M. petrolearium
KW - Polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=85102865122&partnerID=8YFLogxK
U2 - 10.20937/RICA.53837
DO - 10.20937/RICA.53837
M3 - Artículo
AN - SCOPUS:85102865122
SN - 0188-4999
VL - 37
SP - 227
EP - 235
JO - Revista Internacional de Contaminacion Ambiental
JF - Revista Internacional de Contaminacion Ambiental
ER -