TY - JOUR
T1 - Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico
AU - Castro-Escarpulli, G.
AU - Figueras, M. J.
AU - Aguilera-Arreola, G.
AU - Soler, L.
AU - Fernández-Rendón, E.
AU - Aparicio, G. O.
AU - Guarro, J.
AU - Chacón, M. R.
N1 - Funding Information:
This work has been supported by: CGPI 200468 and CGPI 990290 from the Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, México; FIS 99/0944 and FIS 96/0579 from Spanish Ministry of Health; from CIRIT (SGR 1999/00103) and from the Fundació Ciència i Salut. We would like to thank: Graciela Margarita Lugo from the Veterinary Microbiology Laboratory, Escuela Nacional de Ciencias Biológicas (México); Dr. S. Kirov from University of Tasmania Clinical School (Australia); Dr. M. Janda from the Department of Health Service Berkeley (California) and Colección Española de Cultivos Tipo (CECT) for kindly providing isolates.
PY - 2003/7/15
Y1 - 2003/7/15
N2 - A total of 82 strains of presumptive Aeromonas spp. were identified biochemically and genetically (16S rDNA-RFLP). The strains were isolated from 250 samples of frozen fish (Tilapia, Oreochromis niloticus niloticus) purchased in local markets in Mexico City. In the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. In addition, we studied the antimicrobial patterns of those strains. Molecular identification demonstrated that the prevalent species in frozen fish were Aeromonas salmonicida (67.5%) and Aeromonas bestiarum (20.9%), accounting for 88.3% of the isolates, while the other strains belonged to the species Aeromonas veronii (5.2%), Aeromonas encheleia (3.9%) and Aeromonas hydrophila (2.6%). Detection by polymerase chain reaction (PCR) of genes encoding putative virulence factors common in Aeromonas, such as aerolysin/hemolysin, lipases including the glycerophospholipid-cholesterol acyltransferase (GCAT), serine protease and DNases, revealed that they were all common in these strains. Our results showed that first generation quinolones and second and third generation cephalosporins were the drugs with the best antimicrobial effect against Aeromonas spp. In Mexico, there have been few studies on Aeromonas and its putative virulence factors. The present work therefore highlights an important incidence of Aeromonas spp., with virulence potential and antimicrobial resistance, isolated from frozen fish intended for human consumption in Mexico City.
AB - A total of 82 strains of presumptive Aeromonas spp. were identified biochemically and genetically (16S rDNA-RFLP). The strains were isolated from 250 samples of frozen fish (Tilapia, Oreochromis niloticus niloticus) purchased in local markets in Mexico City. In the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. In addition, we studied the antimicrobial patterns of those strains. Molecular identification demonstrated that the prevalent species in frozen fish were Aeromonas salmonicida (67.5%) and Aeromonas bestiarum (20.9%), accounting for 88.3% of the isolates, while the other strains belonged to the species Aeromonas veronii (5.2%), Aeromonas encheleia (3.9%) and Aeromonas hydrophila (2.6%). Detection by polymerase chain reaction (PCR) of genes encoding putative virulence factors common in Aeromonas, such as aerolysin/hemolysin, lipases including the glycerophospholipid-cholesterol acyltransferase (GCAT), serine protease and DNases, revealed that they were all common in these strains. Our results showed that first generation quinolones and second and third generation cephalosporins were the drugs with the best antimicrobial effect against Aeromonas spp. In Mexico, there have been few studies on Aeromonas and its putative virulence factors. The present work therefore highlights an important incidence of Aeromonas spp., with virulence potential and antimicrobial resistance, isolated from frozen fish intended for human consumption in Mexico City.
KW - 16S rRNA-RFLP
KW - Aeromonas
KW - Antimicrobial patterns
KW - Frozen fish
KW - Mexico
KW - Virulence factors
UR - http://www.scopus.com/inward/record.url?scp=0037762827&partnerID=8YFLogxK
U2 - 10.1016/S0168-1605(02)00393-8
DO - 10.1016/S0168-1605(02)00393-8
M3 - Artículo
SN - 0168-1605
VL - 84
SP - 41
EP - 49
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
IS - 1
ER -