TY - JOUR
T1 - CD4+ CD25+ lymphocyte and dendritic cell mobilization with intermediate doses of recombinant human granulocyte colony-stimulating factor in healthy donors
AU - Vela-Ojeda, Jorge
AU - García-Ruiz Esparza, M. A.
AU - Reyes-Maldonado, E.
AU - Jiménez-Zamudio, L.
AU - García-Latorre, E.
AU - Moreno-Lafont, M.
AU - Estrada-García, I.
AU - Mayani, H.
AU - Montiel-Cervantes, L.
AU - Tripp-Villanueva, F.
AU - Ayala-Sánchez, M.
AU - García-León, L. D.
AU - Borbolla-Escoboza, J. R.
PY - 2005/6
Y1 - 2005/6
N2 - We prospectively conducted a quantitative and phenotypic analysis of T, B, natural killer (NK), NKT, type 1 and 2 dendritic cells (DC), and regulatory T cells, before and after mobilization with intermediate doses of granulocyte colony-stimulating factor (G-CSF) (16 μg/kg per day). Between November, 2003, and December, 2004, we collected stem cells from 25 HLA identical sibling donors for allogeneic hematopoietic stem cell transplantation. Before mobilization and 3 h after the fourth and fifth doses of G-CSF, blood samples were taken for blood counts and flow cytometry. The median number of regulatory T cells before and after G-CSF was statistically different (69 ± 41 × 106/L versus 161 ± 159 × 106/L, p < 0.01). We observed a 1.7-fold increase in NK and NKT cells (p < 0.009 and p < 0.02, respectively). DC were mobilized with a 11.5-fold increase in type 2 (p < 0.004) and a 8.5-fold increase in type 1 DC (p < 0.003). The patients received a mean of: 2.2 × 107/kg ± 1.4 × 107/kg of NK cells, 0.95 × 107/kg ± 0.81 × 107/kg of NKT cells, 0.43 × 107/kg ± 0.53 × 107/kg of type 1 DC, 0.3 × 107/kg ± 0.45 × 107/kg of type 2 DC and 1.4 × 10 7/kg ± 1.2 × 107/kg of regulatory T cells. Using intermediate doses of G-CSF, we have demonstrated the mobilization of different lymphocyte subsets, in particular regulatory T cells and DC, which can be expanded later and used in the treatment of cancer and autoimmune diseases.
AB - We prospectively conducted a quantitative and phenotypic analysis of T, B, natural killer (NK), NKT, type 1 and 2 dendritic cells (DC), and regulatory T cells, before and after mobilization with intermediate doses of granulocyte colony-stimulating factor (G-CSF) (16 μg/kg per day). Between November, 2003, and December, 2004, we collected stem cells from 25 HLA identical sibling donors for allogeneic hematopoietic stem cell transplantation. Before mobilization and 3 h after the fourth and fifth doses of G-CSF, blood samples were taken for blood counts and flow cytometry. The median number of regulatory T cells before and after G-CSF was statistically different (69 ± 41 × 106/L versus 161 ± 159 × 106/L, p < 0.01). We observed a 1.7-fold increase in NK and NKT cells (p < 0.009 and p < 0.02, respectively). DC were mobilized with a 11.5-fold increase in type 2 (p < 0.004) and a 8.5-fold increase in type 1 DC (p < 0.003). The patients received a mean of: 2.2 × 107/kg ± 1.4 × 107/kg of NK cells, 0.95 × 107/kg ± 0.81 × 107/kg of NKT cells, 0.43 × 107/kg ± 0.53 × 107/kg of type 1 DC, 0.3 × 107/kg ± 0.45 × 107/kg of type 2 DC and 1.4 × 10 7/kg ± 1.2 × 107/kg of regulatory T cells. Using intermediate doses of G-CSF, we have demonstrated the mobilization of different lymphocyte subsets, in particular regulatory T cells and DC, which can be expanded later and used in the treatment of cancer and autoimmune diseases.
UR - http://www.scopus.com/inward/record.url?scp=21244483508&partnerID=8YFLogxK
U2 - 10.1089/scd.2005.14.310
DO - 10.1089/scd.2005.14.310
M3 - Artículo
SN - 1547-3287
VL - 14
SP - 310
EP - 316
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 3
ER -