TY - JOUR
T1 - Caveolin scaffolding peptide-1 interferes with norepinephrine-induced PLC-β activation in cultured rat vascular smooth muscle cells
AU - Ocharan, Esther
AU - Asbun, Juan
AU - Calzada, Claudia
AU - Mendez, Enrique
AU - Nuñez, Marisol
AU - Medina, Roberta
AU - Suarez, Gabriela
AU - Meaney, Eduardo
AU - Ceballos, Guillermo
PY - 2005/11
Y1 - 2005/11
N2 - Caveolins are a family of integral membrane proteins implicated in various cell functions, including the organization and inactivation of signaling molecules of G protein-coupled receptors. We tested the ability of human caveolin scaffolding peptide-1 (CSP-1) to regulate norepinephrine-(NE) or histamine (HIS)-induced increases on intracellular calcium concentrations ([Ca2+]i). In cultured rat vascular smooth muscle cells (VSMC), CSP-1 inhibited in a concentration-dependent manner NE- and HIS-induced increases in [Ca2+]i. This effect can be explained by the fact that CSP-1 inhibited a common signaling pathway. We tested the ability of this peptide to decrease the activation of PLC-β3 and MAPK. CSP-1 inhibited the expression of the activated form of both enzymes, suggesting a direct effect of the peptide on the signaling cascade. CSP-1 readily enters VSMC in culture, as observed when FITC-conjugated CPS-1 is added to cell culture media. Taken together, these data suggest that CSP-1 blocks the effects of NE and HIS on [Ca2+]i of VSMC by inhibiting the activation of PLC-β3 and MAPK.
AB - Caveolins are a family of integral membrane proteins implicated in various cell functions, including the organization and inactivation of signaling molecules of G protein-coupled receptors. We tested the ability of human caveolin scaffolding peptide-1 (CSP-1) to regulate norepinephrine-(NE) or histamine (HIS)-induced increases on intracellular calcium concentrations ([Ca2+]i). In cultured rat vascular smooth muscle cells (VSMC), CSP-1 inhibited in a concentration-dependent manner NE- and HIS-induced increases in [Ca2+]i. This effect can be explained by the fact that CSP-1 inhibited a common signaling pathway. We tested the ability of this peptide to decrease the activation of PLC-β3 and MAPK. CSP-1 inhibited the expression of the activated form of both enzymes, suggesting a direct effect of the peptide on the signaling cascade. CSP-1 readily enters VSMC in culture, as observed when FITC-conjugated CPS-1 is added to cell culture media. Taken together, these data suggest that CSP-1 blocks the effects of NE and HIS on [Ca2+]i of VSMC by inhibiting the activation of PLC-β3 and MAPK.
KW - Caveolae
KW - Intracellular cell signaling
KW - VSCM
UR - http://www.scopus.com/inward/record.url?scp=27344431597&partnerID=8YFLogxK
U2 - 10.1097/01.fjc.0000181292.34205.ee
DO - 10.1097/01.fjc.0000181292.34205.ee
M3 - Artículo
SN - 0160-2446
VL - 46
SP - 615
EP - 621
JO - Journal of Cardiovascular Pharmacology
JF - Journal of Cardiovascular Pharmacology
IS - 5
ER -