TY - JOUR
T1 - Case report
T2 - Identification of recombinant HCV genotype 1b–2b by viral sequencing in two patients with treatment failure, who responded to re-treatment with sofosbuvir and daclatasvir
AU - Uribe-Noguez, Luis Antonio
AU - Ocaña-Mondragón, Alicia
AU - Mata-Marín, José Antonio
AU - Cázares-Cortázar, Allison
AU - Ribas-Aparicio, Rosa María
AU - Gómez-Torres, María Elena
AU - Gaytán-Martínez, Jesus
AU - Martínez-Rodríguez, María de la Luz
N1 - Publisher Copyright:
© 2018 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases
PY - 2018/11
Y1 - 2018/11
N2 - Hepatitis C virus (HCV) infection is a global health problem. HCV has been classified into seven genotypes and >67 subtypes. Genotyping is necessary to enable selection of appropriate treatments. The commercial molecular techniques currently used do not identify some HCV subtypes, mixed infections and recombinant forms. In this study, the core–E1 and NS5B regions were sequenced and phylogenetically analysed to identify infections by HCV recombinant genotype 1b–2b in two patients who had initially been diagnosed with HCV genotype 2 infection by reverse hybridization with a Versant HCV Genotype 2.0 Assay. Response to treatment was monitored by viral kinetics. Therapeutic failure occurred with initial treatment with PEGylated interferon-α2b and ribavirin, but the use of sofosbuvir and daclatasvir on a re-treatment regimen after reclassification of the infecting virus resulted in a sustained virologic response. The use of a sequencing approach in treatment-naïve infected patients could enable physicians to select the optimal therapy and avoid possible relapses and adverse reactions associated with antiviral therapy.
AB - Hepatitis C virus (HCV) infection is a global health problem. HCV has been classified into seven genotypes and >67 subtypes. Genotyping is necessary to enable selection of appropriate treatments. The commercial molecular techniques currently used do not identify some HCV subtypes, mixed infections and recombinant forms. In this study, the core–E1 and NS5B regions were sequenced and phylogenetically analysed to identify infections by HCV recombinant genotype 1b–2b in two patients who had initially been diagnosed with HCV genotype 2 infection by reverse hybridization with a Versant HCV Genotype 2.0 Assay. Response to treatment was monitored by viral kinetics. Therapeutic failure occurred with initial treatment with PEGylated interferon-α2b and ribavirin, but the use of sofosbuvir and daclatasvir on a re-treatment regimen after reclassification of the infecting virus resulted in a sustained virologic response. The use of a sequencing approach in treatment-naïve infected patients could enable physicians to select the optimal therapy and avoid possible relapses and adverse reactions associated with antiviral therapy.
KW - Genotype 1b-2b
KW - Hepatitis C virus
KW - Phylogenetic analysis
KW - Recombination
KW - Sequencing
UR - http://www.scopus.com/inward/record.url?scp=85047210492&partnerID=8YFLogxK
U2 - 10.1016/j.jiac.2018.04.001
DO - 10.1016/j.jiac.2018.04.001
M3 - Artículo
C2 - 29773440
SN - 1341-321X
VL - 24
SP - 928
EP - 931
JO - Journal of Infection and Chemotherapy
JF - Journal of Infection and Chemotherapy
IS - 11
ER -