TY - JOUR
T1 - Caloric restriction increases free radicals and inducible nitric oxide synthase expression in mice infected with Salmonella typhimurium
AU - Trujillo-Ferrara, José
AU - Campos-Rodríguez, Rafael
AU - Lara-Padilla, Eleazar
AU - Ramírez-Rosales, Daniel
AU - Correa Basurto, José
AU - Miliar Garcia, Angel
AU - Reyna Garfias, Humberto
AU - Zamorano Ulloa, Rafael
AU - Rosales-Hernández, Martha C.
PY - 2011/8
Y1 - 2011/8
N2 - It is well known that CR (caloric restriction) reduces oxidative damage to proteins, lipids and DNA, although the underlying mechanism is unclear. However, information concerning the effect of CR on the host response to infection is sparse. In this study, 6-month-old mice that were fed AL (ad libitum) or with a CR diet were infected with Salmonella serovar Typhimurium. EPR (electron paramagnetic resonance; also known as ESR (electron spin resonance)) was used to identify FRs (free radicals). These results were subsequently correlated with SOD (superoxide dismutase) catalytic activity, iNOS [inducible NOS (nitric oxide synthase) or NOSII] expression and NO (nitric oxide) content. EPR analysis of liver samples demonstrated that there was a higher quantity of FRs and iron-nitrosyl complex in infected mice provided with a CR diet as compared with those on an AL diet, indicating that CR was beneficial by increasing the host response to Salmonella Typhimurium. Furthermore, in infected mice on the CR diet, NOSII expression was higher, NO content was greater and spleen colonization was lower, compared with mice on the AL diet. No changes in SOD activity were detected, indicating that the NO produced participated more in the formation of iron-nitrosyl complexes than peroxynitrite. These results suggest that CR exerts a protective effect against Salmonella Typhimurium infection by increasing NO production.
AB - It is well known that CR (caloric restriction) reduces oxidative damage to proteins, lipids and DNA, although the underlying mechanism is unclear. However, information concerning the effect of CR on the host response to infection is sparse. In this study, 6-month-old mice that were fed AL (ad libitum) or with a CR diet were infected with Salmonella serovar Typhimurium. EPR (electron paramagnetic resonance; also known as ESR (electron spin resonance)) was used to identify FRs (free radicals). These results were subsequently correlated with SOD (superoxide dismutase) catalytic activity, iNOS [inducible NOS (nitric oxide synthase) or NOSII] expression and NO (nitric oxide) content. EPR analysis of liver samples demonstrated that there was a higher quantity of FRs and iron-nitrosyl complex in infected mice provided with a CR diet as compared with those on an AL diet, indicating that CR was beneficial by increasing the host response to Salmonella Typhimurium. Furthermore, in infected mice on the CR diet, NOSII expression was higher, NO content was greater and spleen colonization was lower, compared with mice on the AL diet. No changes in SOD activity were detected, indicating that the NO produced participated more in the formation of iron-nitrosyl complexes than peroxynitrite. These results suggest that CR exerts a protective effect against Salmonella Typhimurium infection by increasing NO production.
KW - Electron paramagnetic resonance (EPR)
KW - Iron-nitrosyl complex
KW - Nitric oxide
KW - Superoxide dismutase (SOD)
UR - http://www.scopus.com/inward/record.url?scp=79960149506&partnerID=8YFLogxK
U2 - 10.1042/BSR20100021
DO - 10.1042/BSR20100021
M3 - Artículo
C2 - 20883207
SN - 0144-8463
VL - 31
SP - 273
EP - 282
JO - Bioscience Reports
JF - Bioscience Reports
IS - 4
ER -