TY - JOUR
T1 - Brownian-particle motion used to characterize mechanical properties of lipid vesicles
AU - Sandoval-Jiménez, Idalia M.
AU - Jacinto-Méndez, Damián
AU - Toscano-Flores, Liliana G.
AU - Carbajal-Tinoco, Mauricio D.
N1 - Publisher Copyright:
© 2020 Author(s).
PY - 2020/1/7
Y1 - 2020/1/7
N2 - We present a method that takes advantage of the Brownian motion of a colloidal particle to experimentally determine the area stretch modulus of giant liposomes in solution, in regard to the continuum mechanics theory of elasticity. The characteristic parameters of the corresponding model are measured by using the three-dimensional version of digital video microscopy. Such an approach makes use of the diffraction pattern generated by fluorescent spheres found below the focal plane of the microscope objective, allowing the spatial location of the sphere and, thus, the reconstruction of its trajectory. When this particle probe is localized in the neighborhood of a bilayer membrane, its motion causes an elastic distortion of the membrane that is quantifiable. More importantly, such deformation is related to the mentioned modulus, whose measured values are consistent with experimental data obtained through well-known techniques.
AB - We present a method that takes advantage of the Brownian motion of a colloidal particle to experimentally determine the area stretch modulus of giant liposomes in solution, in regard to the continuum mechanics theory of elasticity. The characteristic parameters of the corresponding model are measured by using the three-dimensional version of digital video microscopy. Such an approach makes use of the diffraction pattern generated by fluorescent spheres found below the focal plane of the microscope objective, allowing the spatial location of the sphere and, thus, the reconstruction of its trajectory. When this particle probe is localized in the neighborhood of a bilayer membrane, its motion causes an elastic distortion of the membrane that is quantifiable. More importantly, such deformation is related to the mentioned modulus, whose measured values are consistent with experimental data obtained through well-known techniques.
UR - http://www.scopus.com/inward/record.url?scp=85077511457&partnerID=8YFLogxK
U2 - 10.1063/1.5133092
DO - 10.1063/1.5133092
M3 - Artículo
C2 - 31914746
AN - SCOPUS:85077511457
SN - 0021-9606
VL - 152
JO - Journal of Chemical Physics
JF - Journal of Chemical Physics
IS - 1
M1 - 014901
ER -