TY - JOUR
T1 - Broth rheology and morphological analysis of solanum chrysotrichum cultivated in a stirred tank
AU - Trejo-Tapia, Gabriela
AU - Jiménez-Aparicio, Antonio
AU - Villarreal, Luisa
AU - Rodríguez-Monroy, Mario
N1 - Funding Information:
Grants from the Instituto Politécnico Nacional (COFFA/CGEPI-IPN) and Consejo Nacional de Cien-cia y Tecnología (México) supported this work.
PY - 2001
Y1 - 2001
N2 - Solanum chrysotrichum cell cultures were grown in a stirred tank bioreactor and their rheological and morphological behaviour were evaluated. The culture broths exhibited non-Newtonian and shear-thinning characteristics. Pseudoplasticity of the broths was governed by their biomass concentration. The roundness of aggregates measured as the elliptical form factor (EFF) had important changes. At the beginning of the culture the aggregates with an EFF lower than 2 represented 52% of the population, but in stationary phase the proportion increased to 77%. Whereas the size of aggregates did not change 80% of the population had an area lower than 0.1 mm2. Overall, these results indicate that the shape of the aggregate therefore needs to be considered when studying plant broth rheology.
AB - Solanum chrysotrichum cell cultures were grown in a stirred tank bioreactor and their rheological and morphological behaviour were evaluated. The culture broths exhibited non-Newtonian and shear-thinning characteristics. Pseudoplasticity of the broths was governed by their biomass concentration. The roundness of aggregates measured as the elliptical form factor (EFF) had important changes. At the beginning of the culture the aggregates with an EFF lower than 2 represented 52% of the population, but in stationary phase the proportion increased to 77%. Whereas the size of aggregates did not change 80% of the population had an area lower than 0.1 mm2. Overall, these results indicate that the shape of the aggregate therefore needs to be considered when studying plant broth rheology.
KW - Image analysis
KW - Morphology
KW - Rheology
KW - Solanum chrysotrichum
KW - Stirred tank bioreactor
UR - http://www.scopus.com/inward/record.url?scp=0035206792&partnerID=8YFLogxK
U2 - 10.1023/A:1013789918463
DO - 10.1023/A:1013789918463
M3 - Artículo
SN - 0141-5492
VL - 23
SP - 1943
EP - 1946
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 23
ER -