TY - JOUR
T1 - Beta vulgaris L. suspension cultures permeabilized with Triton X-100 retain cell viability and betacyanines production ability
T2 - A digital image analysis study
AU - Trejo-Tapia, Gabriela
AU - Cuevas-Celis, Jonathan
AU - Salcedo-Morales, Guadalupe
AU - Trejo-Espino, José Luis
AU - Arenas-Ocampo, Martha L.
AU - Jiménez-Aparicio, Antonio
PY - 2007/3
Y1 - 2007/3
N2 - The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.
AB - The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (≥70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.
UR - http://www.scopus.com/inward/record.url?scp=34247178569&partnerID=8YFLogxK
U2 - 10.1021/bp0602597
DO - 10.1021/bp0602597
M3 - Artículo
C2 - 17253724
SN - 8756-7938
VL - 23
SP - 359
EP - 363
JO - Biotechnology Progress
JF - Biotechnology Progress
IS - 2
ER -