ATP-binding Cassette Transporter ABCB5b Gene is Expressed with Variability in Malignant Melanoma

Título traducido de la contribución: El gene del Transportador cassette de enlace de ATP ABCB5b está expresado con variabilidad en el melanoma maligno

Vázquez Moctezuma Ismael, Marco A. Meraz-Rios, Guadalupe Cleva Villanueva López, M Magaña, R Martinez Macias, Dolores Javier Sanchez Gonzalez, Francisco García-Sierra, Norma E Herrera-Gonzalez

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Background: Melanoma is a malignant neoplasm with high metastatic disease risk and elevated mortality. Incidence of melanoma varies according to geographic region and genetic background. Epidemiologicals tudies indicate that acral melanoma (AM) is among the most common melanomas in the Mexican population. While extensive studies have identified genes associated with melanoma, little is known about the genes involved in the pathogenesis of AM.
Objective: To compare the gene expressio patterns between primary melanoma andnormal skin.Methods: We used 10 samples of fresh acral melanomas and normal skin for the study of differential gene expression and 22 samples of melanoma for in situ hybridization. Results: We first identified agene that was present in a sample of AM and absent in normal skin. DNA sequencing of thi sdifferentially expressed gene revealed that it corresponded to ABCB5, a gene recently implicated in the regulation of progenitor cell fusion. Furthermore, we detected ABCB5 expression in other melanoma specimens by RT-PCR. We showed that nine out of ten melanomas were positive for ABCB5 while only one melanoma and normal skin samples were negative. All ABCB5 expres sing melanomas had variable gene expression according to in situ hybridization studies,suggesting that the ABCB5 gene may be differentially regulated by individual melanomas
Título traducido de la contribuciónEl gene del Transportador cassette de enlace de ATP ABCB5b está expresado con variabilidad en el melanoma maligno
Idioma originalInglés
Número de artículo4
Páginas (desde-hasta)341-348
Número de páginas8
PublicaciónActas Dermo-Sifilograficas
EstadoPublicada - 16 dic. 2009

Palabras clave

  • melanoma
  • ABCB gene
  • gene expression
  • Differential display

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