TY - JOUR
T1 - Antioxidant and antiproliferative activity of the ethanolic extract of equisetum myriochaetum and molecular docking of its main metabolites (Apigenin, kaempferol, and quercetin) on β-tubulin
AU - Olazarán-Santibañez, Fabián
AU - Rivera, Gildardo
AU - Vanoye-Eligio, Venancio
AU - Mora-Olivo, Arturo
AU - Aguirre-Guzmán, Gabriel
AU - Ramírez-Cabrera, Mónica
AU - Arredondo-Espinoza, Eder
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.
AB - Equisetum myriochaetum is a semi-aquatic plant found on riverbanks that is commonly used in traditional medicine as a diuretic agent. Additionally, the genus Equisetum stands out for its content of the flavonoid kaempferol, a well-known antiproliferative agent. Therefore, in this study, E. myriochaetum ethanolic extract was tested in vitro against a cervical cancer cell line (SiHa). Additionally, the antioxidative activity was evaluated through a 2,2-diphenyl-1-picrilhidrazil (DPPH) assay. Finally, a molecular docking analysis of apigenin, kaempferol, and quercetin on the active site of β-tubulin was performed to investigate their potential mechanism of action. All fractions of E. myriochaetum ethanolic extract showed antioxidative activity. Fraction 14 displayed an antiproliferative capacity with a half maximal inhibitory concentration (IC50) value of 6.78 µg/mL against SiHa cells.
KW - Antioxidative
KW - Antiproliferative
KW - Equisetum myriochaetum
KW - Flavonoids
KW - Kaempferol
KW - Molecular docking
UR - http://www.scopus.com/inward/record.url?scp=85100327687&partnerID=8YFLogxK
U2 - 10.3390/molecules26020443
DO - 10.3390/molecules26020443
M3 - Artículo
C2 - 33466999
AN - SCOPUS:85100327687
SN - 1420-3049
VL - 26
JO - Molecules
JF - Molecules
IS - 2
M1 - 443
ER -
