TY - JOUR
T1 - Antihyperalgesic activity of a mexicanolide isolated from Swietenia humilis extract in nicotinamide-streptozotocin hyperglycemic mice
AU - Ovalle-Magallanes, Berenice
AU - Déciga-Campos, Myrna
AU - Mata, Rachel
N1 - Publisher Copyright:
© 2017
PY - 2017/8
Y1 - 2017/8
N2 - Swietenia humilis Zucc. (Meliaceae) seeds are used in Mexico for the treatment of type 2 diabetes mellitus. Mexicanolides are the main hypoglycemic and antihyperglycemic compounds of the species. This study was conducted to investigate the antihyperalgesic effect of an aqueous extract of the seeds of Swietenia humilis (SHAE) and of mexicanolide 2-hydroxy-destigloyl-6-deoxyswietenine acetate (1), using the formalin test in mice. The antihyperalgesic actions of SHAE and mexicanolide 1, as well as its possible transductional activity, were assessed in nicotinamide-streptozotocin (NA-STZ) hyperglycemic mice. Local injection of SHAE (10–177 μg) and mexicanolide 1 (0.5–3.5 μg) exhibited concentration-dependent antihyperalgesic action in NA-STZ hyperglycemic mice. Ketanserin (6 μg), a 5-HT2A/C receptor antagonist, and flumazenil (6 μg), a GABAA receptor antagonist, abolished the antihyperalgesic effect of mexicanolide 1 (3 μg). On the other hand, naloxone (3 μg), L-arginine (50 μg), and Nω-Nitro-L-arginine methyl ester hydrochloride (150 μg) diminished the antihyperalgesic effect of mexicanolide 1. The aqueous extract of the seeds possesses significant antihyperalgesic action. Compound 1 produces antihyperalgesia through GABAA, 5-HT2A/C and opioid receptors. Also, the nitrergic system is involve in the antihyperalgesic effect of 1. Data obtained with Swietenia humilis Zucc. seeds give evidence of its potential for pain associated with diabetes treatment.
AB - Swietenia humilis Zucc. (Meliaceae) seeds are used in Mexico for the treatment of type 2 diabetes mellitus. Mexicanolides are the main hypoglycemic and antihyperglycemic compounds of the species. This study was conducted to investigate the antihyperalgesic effect of an aqueous extract of the seeds of Swietenia humilis (SHAE) and of mexicanolide 2-hydroxy-destigloyl-6-deoxyswietenine acetate (1), using the formalin test in mice. The antihyperalgesic actions of SHAE and mexicanolide 1, as well as its possible transductional activity, were assessed in nicotinamide-streptozotocin (NA-STZ) hyperglycemic mice. Local injection of SHAE (10–177 μg) and mexicanolide 1 (0.5–3.5 μg) exhibited concentration-dependent antihyperalgesic action in NA-STZ hyperglycemic mice. Ketanserin (6 μg), a 5-HT2A/C receptor antagonist, and flumazenil (6 μg), a GABAA receptor antagonist, abolished the antihyperalgesic effect of mexicanolide 1 (3 μg). On the other hand, naloxone (3 μg), L-arginine (50 μg), and Nω-Nitro-L-arginine methyl ester hydrochloride (150 μg) diminished the antihyperalgesic effect of mexicanolide 1. The aqueous extract of the seeds possesses significant antihyperalgesic action. Compound 1 produces antihyperalgesia through GABAA, 5-HT2A/C and opioid receptors. Also, the nitrergic system is involve in the antihyperalgesic effect of 1. Data obtained with Swietenia humilis Zucc. seeds give evidence of its potential for pain associated with diabetes treatment.
KW - 2-Hydroxy-destigloyl-6-deoxyswietenine acetate
KW - Antihyperalgesia
KW - Diabetes
KW - Formalin
KW - Hyperalgesia
KW - Swietenia humilis
UR - http://www.scopus.com/inward/record.url?scp=85019953543&partnerID=8YFLogxK
U2 - 10.1016/j.biopha.2017.05.073
DO - 10.1016/j.biopha.2017.05.073
M3 - Artículo
C2 - 28551554
SN - 0753-3322
VL - 92
SP - 324
EP - 330
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
ER -