TY - JOUR
T1 - Antibacterial activity evaluation of the nile tilapia Oreochromis niloticus (Linnaeus, 1758) skin mucus, against vibrio bacteria affecting the white shrimp Penaeus vannamei
AU - García-Marciano, Máximo
AU - Apún-Molina, Juan Pablo
AU - Sainz-Hernández, Juan Carlos
AU - Santamaría-Miranda, Apolinar
AU - Medina-Godoy, Sergio
AU - Aguiñaga-Cruz, Jazmín Asusena
N1 - Publisher Copyright:
© 2019, Escuela de Ciencias del Mar. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Shrimp production has been affected by disease outbreaks caused by Vibrio sp. bacteria. Using a polyculture system has been proposed as preventive management, but until now, the mode of action by which the organisms in polyculture obtain some benefits was unknown. Some studies indicate that these benefits are related to the immune system in the skin mucus. The present study aimed to determine the antibacterial potential of skin mucus in two tilapia varieties, Oreochromis sp. (marine adapted) and O. niloticus (freshwater), against Vibrio bacteria that affect the shrimp Penaeus vannamei. Skin mucus extracts were obtained from both varieties of tilapia during spring and winter. The extracts were: acidic, aqueous and two organics. In total, 16 extracts were obtained. During the winter season, no antibacterial activity was detected; however, in the spring, the acidic extract from the marine-adapted tilapia inhibited V. parahaemolyticus. Additionally, V. harveyi was inhibited by the acidic and organic extracts from both varieties. The aim of this study was confirmed: tilapia skin mucus has antibacterial activity against Vibrio bacteria, which depends on the tilapia variety, bacterial strain, season and the concentration of colony forming units. SDS-PAGE revealed a protease of 96 kD present in all extracts, even in those without antibacterial activity.
AB - Shrimp production has been affected by disease outbreaks caused by Vibrio sp. bacteria. Using a polyculture system has been proposed as preventive management, but until now, the mode of action by which the organisms in polyculture obtain some benefits was unknown. Some studies indicate that these benefits are related to the immune system in the skin mucus. The present study aimed to determine the antibacterial potential of skin mucus in two tilapia varieties, Oreochromis sp. (marine adapted) and O. niloticus (freshwater), against Vibrio bacteria that affect the shrimp Penaeus vannamei. Skin mucus extracts were obtained from both varieties of tilapia during spring and winter. The extracts were: acidic, aqueous and two organics. In total, 16 extracts were obtained. During the winter season, no antibacterial activity was detected; however, in the spring, the acidic extract from the marine-adapted tilapia inhibited V. parahaemolyticus. Additionally, V. harveyi was inhibited by the acidic and organic extracts from both varieties. The aim of this study was confirmed: tilapia skin mucus has antibacterial activity against Vibrio bacteria, which depends on the tilapia variety, bacterial strain, season and the concentration of colony forming units. SDS-PAGE revealed a protease of 96 kD present in all extracts, even in those without antibacterial activity.
KW - Bacteria
KW - Immune system
KW - Mucus
KW - Oreochromis niloticus
KW - Tegument
KW - Tilapia
UR - http://www.scopus.com/inward/record.url?scp=85074493593&partnerID=8YFLogxK
U2 - 10.3856/vol47-issue3-fulltext-20
DO - 10.3856/vol47-issue3-fulltext-20
M3 - Artículo
AN - SCOPUS:85074493593
SN - 0718-560X
VL - 47
SP - 580
EP - 585
JO - Latin American Journal of Aquatic Research
JF - Latin American Journal of Aquatic Research
IS - 3
ER -