TY - JOUR
T1 - Anterior subdiaphragmatic vagotomy decreases the IgA antibody response in the small intestines of BALB/c mice
AU - Arciniega-Martínez, Ivonne Maciel
AU - Drago-Serrano, Maria Elisa
AU - Salas-Pimentel, Marisol
AU - Ventura-Juárez, Javier
AU - Reséndiz-Albor, Aldo Arturo
AU - Campos-Rodríguez, Rafael
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/12/15
Y1 - 2019/12/15
N2 - To assess the impact of vagotomy on the IgA-response, male BALB/c mice underwent anterior vagotomy or a sham procedure were sacrificed on day 14 post-operation and the proximal and distal small-gut segments were dissected. In intestinal lavages IgA/IgM antibodies were analysed by ELISA; in Peyer's-patches and lamina-propria cell suspensions the intracellular IgA-associated interleukins (ILs) and pro-inflammatory cytokines in CD4+ T cells were analysed by cytofluorometry. Vagotomy reduced the IgA or increased the IgM antibody concentration in both segments and reduced or increased the lamina- propria CD4+ T cell pro-inflammatory cytokine responses in the distal or proximal segments, respectively. Data show the role of the vagus nerve on the IgA response.
AB - To assess the impact of vagotomy on the IgA-response, male BALB/c mice underwent anterior vagotomy or a sham procedure were sacrificed on day 14 post-operation and the proximal and distal small-gut segments were dissected. In intestinal lavages IgA/IgM antibodies were analysed by ELISA; in Peyer's-patches and lamina-propria cell suspensions the intracellular IgA-associated interleukins (ILs) and pro-inflammatory cytokines in CD4+ T cells were analysed by cytofluorometry. Vagotomy reduced the IgA or increased the IgM antibody concentration in both segments and reduced or increased the lamina- propria CD4+ T cell pro-inflammatory cytokine responses in the distal or proximal segments, respectively. Data show the role of the vagus nerve on the IgA response.
KW - Anterior vagotomy
KW - IgA antibody
KW - IgA plasma cells
KW - Lamina propria
KW - Parasympathetic nervous system
KW - Peyer's patches
KW - Small intestine
UR - http://www.scopus.com/inward/record.url?scp=85074212696&partnerID=8YFLogxK
U2 - 10.1016/j.jneuroim.2019.577072
DO - 10.1016/j.jneuroim.2019.577072
M3 - Artículo
C2 - 31678856
AN - SCOPUS:85074212696
SN - 0165-5728
VL - 337
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
M1 - 577072
ER -