TY - JOUR
T1 - Analysis of morphological characteristics of Solanum chrysotrichum cell suspension cultures
AU - Trejo-Tapia, Gabriela
AU - Hernández-Trujillo, Rosa
AU - Trejo-Espino, José Luis
AU - Jiménez-Aparicio, Antonio
AU - Rodríguez-Monroy, Mario
N1 - Funding Information:
Grants from the Instituto Politecnico Nacional (CGPI-IPN 20010741 and COFAA) and National Council of Science and Technology (CONACyT-39562) supported this work.
PY - 2003/12
Y1 - 2003/12
N2 - Cell size distribution of Solanum chrysotrichum cell suspension cultures was determined using mechanical sieving and an image analysis system. The results were compared using the sieve size (<0.25, 0.25-0.50, 0.5-1.0 and >1.00 mm) as the criterion. Mechanical sieving as well as image analysis showed that S. chrysotrichum cultures developed in shake flasks present a similar tendency to increase in aggregate size as growth persists. However, there are considerable differences in the values of each fraction. Fractions obtained by mechanical sieving were characterized by image analysis demonstrating that an inefficient separation of the cell population exists. The results demonstrate that digital image analysis was more precise than mechanical sieving to describe the cell size distribution changes occurring during cell growth. It was also possible to achieve a total characterization of S. chrysotrichum morphology.
AB - Cell size distribution of Solanum chrysotrichum cell suspension cultures was determined using mechanical sieving and an image analysis system. The results were compared using the sieve size (<0.25, 0.25-0.50, 0.5-1.0 and >1.00 mm) as the criterion. Mechanical sieving as well as image analysis showed that S. chrysotrichum cultures developed in shake flasks present a similar tendency to increase in aggregate size as growth persists. However, there are considerable differences in the values of each fraction. Fractions obtained by mechanical sieving were characterized by image analysis demonstrating that an inefficient separation of the cell population exists. The results demonstrate that digital image analysis was more precise than mechanical sieving to describe the cell size distribution changes occurring during cell growth. It was also possible to achieve a total characterization of S. chrysotrichum morphology.
KW - Cell size distribution
KW - Image analysis
KW - Mechanical sieving
KW - Plant cell morphology
KW - Solanum chrysotrichum
UR - http://www.scopus.com/inward/record.url?scp=0346463038&partnerID=8YFLogxK
U2 - 10.1023/B:WIBI.0000007323.34363.93
DO - 10.1023/B:WIBI.0000007323.34363.93
M3 - Artículo
SN - 0959-3993
VL - 19
SP - 929
EP - 932
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
IS - 9
ER -