TY - JOUR
T1 - Alterations to the contents of plasma membrane structural lipids are associated with structural changes and compartmentalization in platelets in hypertension
AU - García-Rubio, Diana
AU - Rodríguez-Varela, Mario
AU - Martínez-Vieyra, Ivette
AU - de la Mora, M. B.
AU - Méndez-Méndez, Juan Vicente
AU - Durán-Álvarez, Juan C.
AU - Cerecedo, Doris
N1 - Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/12/15
Y1 - 2019/12/15
N2 - Arterial hypertension (HTN) can lead to serious organ damage. Several mechanisms have been implicated in the pathogenesis of HTN including constitutive activation of platelets, which increases the risk of aggregation and clot formation. We recently demonstrated the plasma membranes of platelets from patients with HTN exhibit modified structural and physicochemical properties; Raman and Fourier transform infrared by attenuated total reflectance (FTIR‐ATR) spectroscopy also indicated lipid content and protein structure alterations. This study aimed to precisely quantify the constituents of the main structural phospholipids and cholesterol in the plasma membranes of platelets from patients with HTN and normotensive individuals. We also assessed the consequence of these alterations on platelet structure and function. Liquid chromatography coupled to triple quadrupole mass spectrometry revealed the plasma membranes of HTN platelets contained less cholesterol and phosphatidylcholine, more phosphatidylserine and phosphatidylethanolamine and had similar sphingosine contents. Atomic force microscopy revealed HTN platelets exhibited increased surface roughness and more pleats. Transmission electron microscopy revealed diminution of the internal membranous structures in HTN platelets. Our findings strongly suggest plasma membrane lipid content alterations—including cholesterol depletion—occur in HTN, and these alterations may induce morphological and physiological abnormalities that participate in the functional changes associated with hypertension.
AB - Arterial hypertension (HTN) can lead to serious organ damage. Several mechanisms have been implicated in the pathogenesis of HTN including constitutive activation of platelets, which increases the risk of aggregation and clot formation. We recently demonstrated the plasma membranes of platelets from patients with HTN exhibit modified structural and physicochemical properties; Raman and Fourier transform infrared by attenuated total reflectance (FTIR‐ATR) spectroscopy also indicated lipid content and protein structure alterations. This study aimed to precisely quantify the constituents of the main structural phospholipids and cholesterol in the plasma membranes of platelets from patients with HTN and normotensive individuals. We also assessed the consequence of these alterations on platelet structure and function. Liquid chromatography coupled to triple quadrupole mass spectrometry revealed the plasma membranes of HTN platelets contained less cholesterol and phosphatidylcholine, more phosphatidylserine and phosphatidylethanolamine and had similar sphingosine contents. Atomic force microscopy revealed HTN platelets exhibited increased surface roughness and more pleats. Transmission electron microscopy revealed diminution of the internal membranous structures in HTN platelets. Our findings strongly suggest plasma membrane lipid content alterations—including cholesterol depletion—occur in HTN, and these alterations may induce morphological and physiological abnormalities that participate in the functional changes associated with hypertension.
KW - Activated platelets
KW - Atomic force microscopy
KW - HPLC
KW - LC-MS/MS
KW - Open canalicular system
KW - Raman spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85074887991&partnerID=8YFLogxK
U2 - 10.1016/j.yexcr.2019.111692
DO - 10.1016/j.yexcr.2019.111692
M3 - Artículo
C2 - 31689412
SN - 0014-4827
VL - 385
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
M1 - 111692
ER -