TY - JOUR
T1 - Alginate microcapsules as delivery and protective systems of Bacillus licheniformis in a simulated shrimp's digestive tract
AU - Vega-Carranza, Ana S.
AU - Cervantes-Chávez, José Antonio
AU - Luna-Bárcenas, Gabriel
AU - Luna-González, Antonio
AU - Diarte-Plata, Genaro
AU - Nava-Mendoza, Rufino
AU - Rodríguez-Morales, José Alberto
AU - Escamilla-Montes, Ruth
AU - Pool, Héctor
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/7/15
Y1 - 2021/7/15
N2 - This work aims to encapsulate Bacillus licheniformis, a marine probiotic, in alginate microparticles (AMPs), and the evaluation of its controlled and targeted release within a simulated shrimp digestive tract (DT). The encapsulation process was carried out using the ionic gelation technique. Both free and bacteria-loaded AMPs were physicochemically characterized by size, morphology, surface electrical charge, the survival, and the number of encapsulated bacteria after the encapsulation process, and the bacterial survival after 40-days of storage (at 4 °C and 25 °C). The in vitro release and survival studies of the bacteria were carried out using a protocol developed in our laboratory by implementing buffers of dissected organs from shrimp's DT. Results indicated that microparticles with an average size of 172–185 μm and negatively charged (−16.77 and − 17.66 mV, respectively) were obtained after using the ionic gelation method. The bacterial survival and encapsulation efficiency showed high cell viability and yield above 99%. Stability studies showed that the best storage temperature was 4 °C, in which it remained almost 100% of the bacteria viable for 15 days; however, cell viability declined to 55% survival after 30 days of storage at this temperature. Regardless of the cell viability reduction after 30 days, there are enough viable bacteria cells to be considered as a probiotic product. Release and survival studies showed that alginate particles had a protective effect on bacteria by keeping ca. 51.29% of viable probiotic within the shrimp intestine; in contrast, free bacteria only reached the shrimp intestine ca. 27.16% viable. Our results suggest that microparticles can be produced by a low-cost method that could ultimately benefit shrimp farming in a near future.
AB - This work aims to encapsulate Bacillus licheniformis, a marine probiotic, in alginate microparticles (AMPs), and the evaluation of its controlled and targeted release within a simulated shrimp digestive tract (DT). The encapsulation process was carried out using the ionic gelation technique. Both free and bacteria-loaded AMPs were physicochemically characterized by size, morphology, surface electrical charge, the survival, and the number of encapsulated bacteria after the encapsulation process, and the bacterial survival after 40-days of storage (at 4 °C and 25 °C). The in vitro release and survival studies of the bacteria were carried out using a protocol developed in our laboratory by implementing buffers of dissected organs from shrimp's DT. Results indicated that microparticles with an average size of 172–185 μm and negatively charged (−16.77 and − 17.66 mV, respectively) were obtained after using the ionic gelation method. The bacterial survival and encapsulation efficiency showed high cell viability and yield above 99%. Stability studies showed that the best storage temperature was 4 °C, in which it remained almost 100% of the bacteria viable for 15 days; however, cell viability declined to 55% survival after 30 days of storage at this temperature. Regardless of the cell viability reduction after 30 days, there are enough viable bacteria cells to be considered as a probiotic product. Release and survival studies showed that alginate particles had a protective effect on bacteria by keeping ca. 51.29% of viable probiotic within the shrimp intestine; in contrast, free bacteria only reached the shrimp intestine ca. 27.16% viable. Our results suggest that microparticles can be produced by a low-cost method that could ultimately benefit shrimp farming in a near future.
KW - Marine probiotics
KW - Microparticulate polysaccharides
KW - Shrimp's mimicked gastrointestinal tract
KW - Targeted delivery systems
UR - http://www.scopus.com/inward/record.url?scp=85103385492&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2021.736675
DO - 10.1016/j.aquaculture.2021.736675
M3 - Artículo
AN - SCOPUS:85103385492
SN - 0044-8486
VL - 540
JO - Aquaculture
JF - Aquaculture
M1 - 736675
ER -