TY - JOUR
T1 - Aeromonas hydrophila clinical and environmental ecotypes as revealed by genetic diversity and virulence genes
AU - Aguilera-Arreola, Ma Guadalupe
AU - Hernández-Rodríguez, César
AU - Zúñiga, Gerardo
AU - Figueras, María José
AU - Castro-Escarpulli, Graciela
N1 - Funding Information:
M.G.A.A. is a fellow from CONACyT. GCE, GZB and CHR received COFAA and EDD, IPN supports. This work was supported by grants from CGPI 20030305 IPN, Mexico and Fundació Ciència i Salut, Ministerio de Sanidad y Consumo (FIS03/1183), España. We thank Dr. M. Janda from the Department of Health Service Berkeley (CA), Colección Española de Cultivos Tipo, MSP Elizabeth Fernández-Rendón from Departamento de Microbiologı́a, ENCB-IPN and Dra. Miroslava Sánchez from Laboratorio de Salud Pública, Hidalgo, Mexico, for providing isolates.
PY - 2005/1/15
Y1 - 2005/1/15
N2 - Aeromonas hydrophila strains recovered from clinical samples and ambient sources were phenotypically and genetically identified. In addition, the distribution of putative virulence factors was assayed. To determine the genetic diversity of these strains, random amplification of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC)-PCR markers were used. The discriminatory ability of the techniques, using Simpson's index, was 0.96 for both methods. The most consistent dendrogram was obtained when RAPD and ERIC data were combined. The genetic diversity revealed a high intra-specific genetic diversity (h = 0.364 ± 0.024 and I = 0.538 ± 0.030). The strains showed a tendency to cluster according to their origin of isolation (best-cut test 0.80 and bootstrap values >50%). The present study demonstrates and quantifies the high intra-specific diversity within this species and reveals a clear differentiation of strains according to their ecological origin. The distribution of virulence-related genes confirm that A. hydrophila is a genetically heterogeneous species that harbour ecotypes which have different pathogenic potential to human and other animals.
AB - Aeromonas hydrophila strains recovered from clinical samples and ambient sources were phenotypically and genetically identified. In addition, the distribution of putative virulence factors was assayed. To determine the genetic diversity of these strains, random amplification of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC)-PCR markers were used. The discriminatory ability of the techniques, using Simpson's index, was 0.96 for both methods. The most consistent dendrogram was obtained when RAPD and ERIC data were combined. The genetic diversity revealed a high intra-specific genetic diversity (h = 0.364 ± 0.024 and I = 0.538 ± 0.030). The strains showed a tendency to cluster according to their origin of isolation (best-cut test 0.80 and bootstrap values >50%). The present study demonstrates and quantifies the high intra-specific diversity within this species and reveals a clear differentiation of strains according to their ecological origin. The distribution of virulence-related genes confirm that A. hydrophila is a genetically heterogeneous species that harbour ecotypes which have different pathogenic potential to human and other animals.
KW - Aeromonas hydrophila
KW - Genetic diversity
UR - http://www.scopus.com/inward/record.url?scp=11144261040&partnerID=8YFLogxK
U2 - 10.1016/j.femsle.2004.11.011
DO - 10.1016/j.femsle.2004.11.011
M3 - Artículo
SN - 0378-1097
VL - 242
SP - 231
EP - 240
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -