TY - JOUR
T1 - Acetonic and methanolic extracts of heterotheca inuloides, and quercetin, decrease CCloxidative stress in several rat tissues
AU - Coballase-Urrutia, Elvia
AU - Pedraza-Chaverri, José
AU - Cárdenas-Rodríguez, Noemí
AU - Huerta-Gertrudis, Bernardino
AU - García-Cruz, Mercedes Edna
AU - Montesinos-Correa, Hortencia
AU - Sánchez-González, Dolores Javier
AU - Camacho-Carranza, Rafael
AU - Espinosa-Aguirre, Jesús Javier
PY - 2013
Y1 - 2013
N2 - The present study was designed to test the hypothesis that the acetonic and methanolic extracts of H. inuloides prevent carbon tetrachloride-(CCl induced oxidative stress in vital tissues. Pretreatment with both H. inuloides extracts or quercetin attenuated the increase in serum activity of alkaline phosphatase (ALP), total bilirubin (BB), creatinine (CRE), and creatine kinase (CK), and impeded the decrease of γ-globulin (γ-GLOB) and albumin (ALB) observed in CClinduced tissue injury. The protective effect was confirmed by histological analysis with hematoxylin-eosin and periodic acid/Schiff's reagent. Level of lipid peroxidation was higher in the organs of rats exposed to CClthan in those of the animals treated with Heterohteca extracts or quercetin, and these showed levels similar to the untreated group. Pretreatment of animals with either of the extracts or quercetin also prevented the increase of 4-hydroxynonenal and 3-nitrotyrosine. Pretreatment with the plant extracts or quercetin attenuated CCltoxic effects on the activity of several antioxidant enzymes. The present results strongly suggest that the chemopreventive effect of the extracts used and quercetin, against CCltoxicity, is associated with their antioxidant properties and corroborated previous results obtained in liver tissue.
AB - The present study was designed to test the hypothesis that the acetonic and methanolic extracts of H. inuloides prevent carbon tetrachloride-(CCl induced oxidative stress in vital tissues. Pretreatment with both H. inuloides extracts or quercetin attenuated the increase in serum activity of alkaline phosphatase (ALP), total bilirubin (BB), creatinine (CRE), and creatine kinase (CK), and impeded the decrease of γ-globulin (γ-GLOB) and albumin (ALB) observed in CClinduced tissue injury. The protective effect was confirmed by histological analysis with hematoxylin-eosin and periodic acid/Schiff's reagent. Level of lipid peroxidation was higher in the organs of rats exposed to CClthan in those of the animals treated with Heterohteca extracts or quercetin, and these showed levels similar to the untreated group. Pretreatment of animals with either of the extracts or quercetin also prevented the increase of 4-hydroxynonenal and 3-nitrotyrosine. Pretreatment with the plant extracts or quercetin attenuated CCltoxic effects on the activity of several antioxidant enzymes. The present results strongly suggest that the chemopreventive effect of the extracts used and quercetin, against CCltoxicity, is associated with their antioxidant properties and corroborated previous results obtained in liver tissue.
UR - http://www.scopus.com/inward/record.url?scp=84873377436&partnerID=8YFLogxK
U2 - 10.1155/2013/659165
DO - 10.1155/2013/659165
M3 - Artículo
C2 - 23365610
SN - 1741-427X
VL - 2013
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 659165
ER -