TY - JOUR
T1 - A simple and rapid method for determination of fluconazole in human plasma samples by high-performance liquid chromatography
AU - Flores-Murrieta, F. J.
AU - Granados-Soto, V.
AU - Hong, E.
N1 - Funding Information:
The authors are indebted to Dr. J. Herrera for providing the plasma samples and Mr. A. Franco for drawings. This work was partially supported by Laboratorios Senosiain, S . A de C.V.
PY - 1994/10/1
Y1 - 1994/10/1
N2 - A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans.
AB - A rapid and simple method for determination of fluconazole in plasma samples by high performance liquid chromatography was developed. The method includes a single extraction of alkalinized plasma with ethyl acetate. Plasma extracts were analyzed on a reverse-phase column eluted with a mixture of acetonitrile and 0.05 M sodium monohydrogen phosphate (pH 4) and detected by absorbance at 210 nm. Retention times for fluconazole and the internal standard were 7 and 13 min respectively. The method was linear in the range of 0.1 to 5 fig/ml and the detection limit of the method was 20 ng/ml. This method is suitable for determination of fluconazole after administration of the drug at therapeutic doses, and it could be used in pharmacokinetic studies of the drug in humans.
UR - http://www.scopus.com/inward/record.url?scp=0028173183&partnerID=8YFLogxK
U2 - 10.1080/10826079408013995
DO - 10.1080/10826079408013995
M3 - Artículo
SN - 0148-3919
VL - 17
SP - 3803
EP - 3811
JO - Journal of Liquid Chromatography
JF - Journal of Liquid Chromatography
IS - 17
ER -