TY - JOUR
T1 - A novel method for the isolation of mycobacterial DNA
AU - Gonzalez-Y-Merchand, Jorge A.
AU - Estrada-Garcia, Iris
AU - Colston, M. Joseph
AU - Cox, Robert A.
PY - 1996/1/1
Y1 - 1996/1/1
N2 - DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to -70°C, then incubated at 65°C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 X 109 or more cells) including Mycobacterium neoaurum, M. fortuitum, M. phlei and M. smegmatis. Each of the species was shown to have two ribosomal RNA operons per genome, and preliminary evidence was obtained which suggests that one of these operons is homologous with one of the operons of M. smegmatis.
AB - DNA was isolated from mycobacteria by a simplified procedure. Cells were suspended in 6 M guanidinium chloride, the suspension was cooled to -70°C, then incubated at 65°C for 10 min, cooled in ice, deproteinized by chloroform and DNA was recovered from the supernatant. The procedure was used to obtain DNA from several mycobacteria (1 X 109 or more cells) including Mycobacterium neoaurum, M. fortuitum, M. phlei and M. smegmatis. Each of the species was shown to have two ribosomal RNA operons per genome, and preliminary evidence was obtained which suggests that one of these operons is homologous with one of the operons of M. smegmatis.
KW - DNA isolation
KW - Mycobacteria
KW - rRNA operon
UR - http://www.scopus.com/inward/record.url?scp=0030064660&partnerID=8YFLogxK
U2 - 10.1016/0378-1097(95)00432-7
DO - 10.1016/0378-1097(95)00432-7
M3 - Artículo
SN - 0378-1097
VL - 135
SP - 71
EP - 77
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 1
ER -