TY - JOUR
T1 - A novel culture medium designed for the simultaneous enhancement of biomass and lipid production by Chlorella vulgaris UTEX 26
AU - Ramírez-López, Citlally
AU - Chairez, Isaac
AU - Fernández-Linares, Luis
N1 - Publisher Copyright:
© 2016 Elsevier Ltd.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - A novel culture medium to enhance the biomass and lipid production simultaneously by Chlorella vulgaris UTEX 26 was designed in three stages of optimization. Initially, a culture medium was inferred applying the response surface method to adjust six factors [NaNO3, NH4HCO3, MgSO4·7H2O, KH2PO4, K2HPO4 and (NH4)2HPO4], which were selected on the basement of BBM (Bold's Basal Medium) and HAMGM (Highly Assimilable Minimal Growth Medium) culture media. Afterwards, the nitrogen source compound was optimized to reduce both, ammonium and nitrate concentrations. As result of the optimization process, the proposed culture medium improved 40% the biomass (0.73 g L-1) compared with the BBM medium and 85% the lipid concentration (281 mg L-1), with respect to HAMGM medium. Some culture media components concentrations were reduced up to 50%. Gas chromatography analysis revealed that C16:0, C18:0, C18:1, C18:2 and C18:3 were the major fatty acids produced by C. vulgaris UTEX 26.
AB - A novel culture medium to enhance the biomass and lipid production simultaneously by Chlorella vulgaris UTEX 26 was designed in three stages of optimization. Initially, a culture medium was inferred applying the response surface method to adjust six factors [NaNO3, NH4HCO3, MgSO4·7H2O, KH2PO4, K2HPO4 and (NH4)2HPO4], which were selected on the basement of BBM (Bold's Basal Medium) and HAMGM (Highly Assimilable Minimal Growth Medium) culture media. Afterwards, the nitrogen source compound was optimized to reduce both, ammonium and nitrate concentrations. As result of the optimization process, the proposed culture medium improved 40% the biomass (0.73 g L-1) compared with the BBM medium and 85% the lipid concentration (281 mg L-1), with respect to HAMGM medium. Some culture media components concentrations were reduced up to 50%. Gas chromatography analysis revealed that C16:0, C18:0, C18:1, C18:2 and C18:3 were the major fatty acids produced by C. vulgaris UTEX 26.
KW - Ammonium bicarbonate
KW - Chlorella vulgaris
KW - Culture medium
KW - Fatty acids
KW - Response surface method
UR - http://www.scopus.com/inward/record.url?scp=84963604732&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2016.04.051
DO - 10.1016/j.biortech.2016.04.051
M3 - Artículo
C2 - 27099946
SN - 0960-8524
VL - 212
SP - 207
EP - 216
JO - Bioresource Technology
JF - Bioresource Technology
ER -