@article{9b46980164514de792ef5b464a36e7d8,
title = "A biorefinery from Nannochloropsis sp. microalga - Extraction of oils and pigments. Production of biohydrogen from the leftover biomass",
abstract = "The microalga Nannochloropsis sp. was used in this study, in a biorefinery context, as biomass feedstock for the production of fatty acids for biodiesel, biohydrogen and high added-value compounds. The microalgal biomass, which has a high lipid and pigment content (mainly carotenoids), was submitted to supercritical CO2 extraction. The temperature, pressure and solvent flow-rate were evaluated to check their effect on the extraction yield. The best operational conditions to extract 33glipids/100gdrybiomass were found to be at 40°C, 300bar and a CO2 flow-rate of 0.62g/min. The effect of adding a co-solvent (ethanol) was also studied. When supercritical CO2 doped with 20% (w/w) ethanol was used, it was possible to extract 45glipids/100gdrybiomass of lipids and recover 70% of the pigments. Furthermore, the remaining biomass after extraction was effectively used as feedstock to produce biohydrogen through dark fermentation by Enterobacter aerogenes resulting in a hydrogen production yield of 60.6mL/gdrybiomass.",
keywords = "Biohydrogen, Biorefinery, Carotenoids, Nannochloropsis, Oils",
author = "Nobre, {B. P.} and F. Villalobos and Barrag{\'a}n, {B. E.} and Oliveira, {A. C.} and Batista, {A. P.} and Marques, {P. A.S.S.} and Mendes, {R. L.} and H. Sovov{\'a} and Palavra, {A. F.} and L. Gouveia",
note = "Funding Information: This research was part of “Microalgae as a sustainable raw material for biofuel production (Biodiesel, Bioethanol, Bio-H 2 and Biogas)” (PTDC/AAC-AMB/100354/2008), a project sponsored by the Portuguese Foundation for Science and Technology (Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia – FCT) . Beatriz P. Nobre thanks FCT for the research grant (SFRH/BPD/42004/2007). F. Villalobos thank IPN (Project SIP 20110775) and CONACyT for a graduate scholarship. The authors would also like to thank Dr. Stephanie Seddon-Brown for the English proofreading and the reviewers for their valuable comments that have improved the manuscript quality. ",
year = "2013",
month = may,
doi = "10.1016/j.biortech.2012.11.084",
language = "Ingl{\'e}s",
volume = "135",
pages = "128--136",
journal = "Bioresource Technology",
issn = "0960-8524",
}