TY - JOUR
T1 - α(1D)-Adrenoceptors contribute to the neurogenic vasopressor response in pithed rats
AU - Castillo, E. F.
AU - López, R. M.
AU - Rodríguez-Silverio, J.
AU - Bobadilla, R. A.
AU - Castillo, C.
PY - 1998
Y1 - 1998
N2 - The aim of the present study was to assess the role of vascular α(1D)- adrenoceptors in the sympathetic vasopressor response in vivo. Specifically, we evaluated the effect of a selective α(1D)-adrenoceptor antagonist, BMY 7378 (8-(2-(4-(2-methoxyphenyl)-I-piperazinyl)ethyl)-8-azaspiro(4,5)decane- 7,9-dione 2HCl), on the vasopressor response induced by preganglionic (T7- T9) sympathetic stimulation in the pithed rat. The vasopressor response was dose-dependently sensitive to inhibition by intravenous BMY 7378 (0.1, 0.31, 1 and 3.1 mg/kg), doses of 1 and 3.1 mg/kg being equally effective. Like BMY 7378, 5-methylurapidil (0.1, 0.31, 1 and 3.1 mg/kg) antagonized the vasopressor response to spinal stimulation; doses of 1 and 3.1 mg/kg were also equally effective. In combination experiments, BMY 7378 (1 mg/kg, iv) and the α(1A)-adrenoceptor antagonist, 5-methylurapidil (1 mg/kg, iv), showed an additive effect. The present results demonstrate that the α(1D)- adrenoceptor subtype plays an important role in the pressor response to sympathetic nerve stimulation in the pithed rat, and confirm the participation of the α(1A)-adrenoceptor subtype in the same response.
AB - The aim of the present study was to assess the role of vascular α(1D)- adrenoceptors in the sympathetic vasopressor response in vivo. Specifically, we evaluated the effect of a selective α(1D)-adrenoceptor antagonist, BMY 7378 (8-(2-(4-(2-methoxyphenyl)-I-piperazinyl)ethyl)-8-azaspiro(4,5)decane- 7,9-dione 2HCl), on the vasopressor response induced by preganglionic (T7- T9) sympathetic stimulation in the pithed rat. The vasopressor response was dose-dependently sensitive to inhibition by intravenous BMY 7378 (0.1, 0.31, 1 and 3.1 mg/kg), doses of 1 and 3.1 mg/kg being equally effective. Like BMY 7378, 5-methylurapidil (0.1, 0.31, 1 and 3.1 mg/kg) antagonized the vasopressor response to spinal stimulation; doses of 1 and 3.1 mg/kg were also equally effective. In combination experiments, BMY 7378 (1 mg/kg, iv) and the α(1A)-adrenoceptor antagonist, 5-methylurapidil (1 mg/kg, iv), showed an additive effect. The present results demonstrate that the α(1D)- adrenoceptor subtype plays an important role in the pressor response to sympathetic nerve stimulation in the pithed rat, and confirm the participation of the α(1A)-adrenoceptor subtype in the same response.
KW - 5-methylurapidil
KW - BMY 7378
KW - Pithed rats
KW - Pressor response
KW - α(1D)-adrenoceptors
UR - http://www.scopus.com/inward/record.url?scp=0344717977&partnerID=8YFLogxK
U2 - 10.1111/j.1472-8206.1998.tb00990.x
DO - 10.1111/j.1472-8206.1998.tb00990.x
M3 - Artículo
SN - 0767-3981
VL - 12
SP - 584
EP - 589
JO - Fundamental and Clinical Pharmacology
JF - Fundamental and Clinical Pharmacology
IS - 6
ER -