Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus

Marcos F. Hernández-Robles, Ana K. Álvarez-Contreras, Patricia Juárez-García, Iván Natividad-Bonifacio, Everardo Curiel-Quesada, Carlos Vázquez-Salinas, Elsa Irma Quiñones-Ramírez

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11 Citations (Scopus)

Abstract

© 2016, Sociedad Espanola de Microbiologia. All rights reserved. Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule. Strains displayed swimming and swarming motility and 93.3% of them produced siderophores. Several genes encoding virulence factors were detected using PCR amplification. These included proA, wza, vopD, vopB, hcp, vasH and vgrG genes, which were present in all strains. Other genes had a variable representation: tdh (86.6%), lafA (96.6%), pvsA (62%) and pvuA (16%). The trh gene could not be amplified from any of the strains. The antimicrobial resistance profile revealed that more than 90% of the strains were resistant to beta-lactams antibiotics, 60% to cephalotin, 45% to amikacin, 16% to cephotaxime, and 10% to pefloxacin, while 100% were susceptible to ceftriaxone. The V. alginolyticus strains isolated from oysters showed multiple resistance to antibiotics and several virulence factors described in well-characterized pathogenic vibrios.
Original languageAmerican English
Pages (from-to)191-198
Number of pages171
JournalInternational Microbiology
DOIs
StatePublished - 1 Jan 2016

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Vibrio alginolyticus
Virulence Factors
Ostreidae
Genes
Pefloxacin
Siderophores
Vibrio
Amikacin
Ceftriaxone
Cholera
beta-Lactams
Microbial Drug Resistance
Hemolysis
Mexico
Capsules
Virulence
Anti-Bacterial Agents
Food
Polymerase Chain Reaction

Cite this

Hernández-Robles, Marcos F. ; Álvarez-Contreras, Ana K. ; Juárez-García, Patricia ; Natividad-Bonifacio, Iván ; Curiel-Quesada, Everardo ; Vázquez-Salinas, Carlos ; Quiñones-Ramírez, Elsa Irma. / Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus. In: International Microbiology. 2016 ; pp. 191-198.
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abstract = "{\circledC} 2016, Sociedad Espanola de Microbiologia. All rights reserved. Vibrio alginolyticus has acquired increasing importance because this microorganism may be pathogenic to aquatic animals and humans. It has been reported that some V. alginolyticus strains carry virulence genes derived from pathogenic V. cholerae and V. parahaemolyticus strains. In this work V. alginolyticus was isolated from oyster samples acquired from a food-market in Mexico City. Thirty isolates were identified as V. alginolitycus. Strains showed β-haemolysis and proteolytic activity and produced a capsule. Strains displayed swimming and swarming motility and 93.3{\%} of them produced siderophores. Several genes encoding virulence factors were detected using PCR amplification. These included proA, wza, vopD, vopB, hcp, vasH and vgrG genes, which were present in all strains. Other genes had a variable representation: tdh (86.6{\%}), lafA (96.6{\%}), pvsA (62{\%}) and pvuA (16{\%}). The trh gene could not be amplified from any of the strains. The antimicrobial resistance profile revealed that more than 90{\%} of the strains were resistant to beta-lactams antibiotics, 60{\%} to cephalotin, 45{\%} to amikacin, 16{\%} to cephotaxime, and 10{\%} to pefloxacin, while 100{\%} were susceptible to ceftriaxone. The V. alginolyticus strains isolated from oysters showed multiple resistance to antibiotics and several virulence factors described in well-characterized pathogenic vibrios.",
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Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus. / Hernández-Robles, Marcos F.; Álvarez-Contreras, Ana K.; Juárez-García, Patricia; Natividad-Bonifacio, Iván; Curiel-Quesada, Everardo; Vázquez-Salinas, Carlos; Quiñones-Ramírez, Elsa Irma.

In: International Microbiology, 01.01.2016, p. 191-198.

Research output: Contribution to journalArticle

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