TY - JOUR
T1 - Variation in protein and amino acids content among landraces of common bean (Phaseolus vulgaris L.)
AU - Flores-Sosa, Ángel Ramón
AU - Aquino-Bolaños, Elia Nora
AU - Cardador-Martínez, Anaberta
AU - Chávez-Servia, José Luis
AU - Vera-Guzmán, Araceli Minerva
AU - Carrillo-Rodríguez, José Cruz
AU - Jiménez, Jimena Esther Alba
N1 - Publisher Copyright:
© 2020. All Rights Reserved.
PY - 2020
Y1 - 2020
N2 - Native bean populations (Phaseolus vulgaris L.) provide bioactive and nutrient compounds; however, their amino acid profiles are unknown. Therefore, the aim of this study is to evaluate the protein content and amino acid profile of 46 native bean populations cultivated by small farmers in Oaxaca, Mexico, and compare them with that of commercial beans. Through high-performance liquid chromatography (HPLC), 16 amino acids were identified and quantified in all samples. The region of origin influenced the concentrations of amino acids. The Santa Lucia Miahuatlan populations stood out for their high content of isoleucine, threonine, methionine, arginine, serine, alanine, tyrosine, and cysteine. Amino acid content showed high variability among the populations; accessions labeled as FSLM22, FSLM27, FSLM28, and FSLM32 were enriched in aliphatic, hydroxylated, aromatic, acidic, and basic amino acids, while the FSLM14, FSLM17, and FSLM18 populations had the highest concentrations of sulfur amino acids. The FSLM01, FSLM22, FSLM27, FSLM28, FSLM30, and FSLM32 populations frequently displayed the highest concentrations of essential amino acids. The findings show that samples of native populations are highly variable in amino acid content due to the genetic characteristics of cultivated beans, environmental and agroecological influences, and crop management by farmers. The beans populations stood out can be used for direct use or a basis for the initiation of a breeding program.
AB - Native bean populations (Phaseolus vulgaris L.) provide bioactive and nutrient compounds; however, their amino acid profiles are unknown. Therefore, the aim of this study is to evaluate the protein content and amino acid profile of 46 native bean populations cultivated by small farmers in Oaxaca, Mexico, and compare them with that of commercial beans. Through high-performance liquid chromatography (HPLC), 16 amino acids were identified and quantified in all samples. The region of origin influenced the concentrations of amino acids. The Santa Lucia Miahuatlan populations stood out for their high content of isoleucine, threonine, methionine, arginine, serine, alanine, tyrosine, and cysteine. Amino acid content showed high variability among the populations; accessions labeled as FSLM22, FSLM27, FSLM28, and FSLM32 were enriched in aliphatic, hydroxylated, aromatic, acidic, and basic amino acids, while the FSLM14, FSLM17, and FSLM18 populations had the highest concentrations of sulfur amino acids. The FSLM01, FSLM22, FSLM27, FSLM28, FSLM30, and FSLM32 populations frequently displayed the highest concentrations of essential amino acids. The findings show that samples of native populations are highly variable in amino acid content due to the genetic characteristics of cultivated beans, environmental and agroecological influences, and crop management by farmers. The beans populations stood out can be used for direct use or a basis for the initiation of a breeding program.
KW - Amino acids
KW - HPLC
KW - Landraces
KW - Phaseolus
UR - http://www.scopus.com/inward/record.url?scp=85101208344&partnerID=8YFLogxK
U2 - 10.9755/ejfa.2020.v32.i10.2175
DO - 10.9755/ejfa.2020.v32.i10.2175
M3 - Artículo
AN - SCOPUS:85101208344
SN - 2079-052X
VL - 32
SP - 750
EP - 760
JO - Emirates Journal of Food and Agriculture
JF - Emirates Journal of Food and Agriculture
IS - 10
ER -