TY - JOUR
T1 - Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.
AU - López-Laredo, Alma R.
AU - Gómez-Aguirre, Yenny Adriana
AU - Medina-Pérez, Virginia
AU - Salcedo-Morales, Guadalupe
AU - Sepúlveda-Jiménez, Gabriela
AU - Trejo-Tapia, Gabriela
N1 - Funding Information:
Acknowledgments This research was financed by the Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (SIP-IPN, Grants 20110153 and 20120745) and Fondo Mixto de Fomento a la Investigación Científica y Tecnológica CONACYT-Gobierno del Estado de Morelos (MOR-2007-C01-79409). ARLL, GSM, GSJ and GTT thank EDI and/or SIBE-IPN. YAGA is indebted to CONACyT for the doctoral fellowship awarded and PIFI-IPN.
PY - 2012/11
Y1 - 2012/11
N2 - The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0. 05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37. 95 mg gallic acid equiv. g -1 dry weight, P < 0. 05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0. 05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e. g. inflammation, tumors).
AB - The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0. 05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37. 95 mg gallic acid equiv. g -1 dry weight, P < 0. 05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0. 05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e. g. inflammation, tumors).
KW - Antioxidant compounds
KW - Castilleja tenuiflora
KW - Flavonoids
KW - Free-radical scavenging
KW - Phenylethanoids
KW - Reducing power
UR - http://www.scopus.com/inward/record.url?scp=84867626968&partnerID=8YFLogxK
U2 - 10.1007/s11738-012-1025-8
DO - 10.1007/s11738-012-1025-8
M3 - Artículo
SN - 0137-5881
VL - 34
SP - 2435
EP - 2442
JO - Acta Physiologiae Plantarum
JF - Acta Physiologiae Plantarum
IS - 6
ER -