vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia

Gloria Fernández-Tilapa, Jazmín Axinecuilteco-Hilera, Silvia Giono-Cerezo, Dinorah Nashely Martínez-Carrillo, Berenice Illades-Aguiar, Adolfo Román-Román

Research output: Contribution to journalArticle

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Abstract

Objective: The aims of this research were to determine the prevalence of Helicobacter pylori and its vacA genotypes in oral cavity in persons without dyspepsia and to establish the association between the presence of H. pylori in oral cavity and oral hygiene. The seroprevalence of anti-H. pylori antibodies and its associated factors were analyzed too. Study design: For the study, 200 adults without dyspepsia symptoms were selected. Dental plaque and saliva samples from each subject were obtained. H. pylori detection in oral samples was carried out by polymerase chain reaction (PCR) and for vacA genotyping a semi-nested and nested PCR was used. The enzyme-linked immunosorbent assay (ELISA) was used to detect anti-H. pylori IgG and IgM. The data were analyzed with Chi square and Fisher exact test and the statistical significance was set to 0.05. Results: Of 200 subjects tested, 124 (62%) were seropositive. H. pylori was detected in the oral cavity of 34 subjects (17%) and vacA allelotypes were typified in 12 of those samples. The s1 allele was detected in 8 (66.7%) samples and in one of them m1 and m2 alleles were found. In four subjects vacA m1 subtypes were found and in two of those both m1 and m2 alleles were detected. The prevalence of H. pylori in oral cavity was higher (l8.5%) among seropositive subjects compared with seronegative persons. No association was found between the presence of H. pylori and oral hygiene habits. Conclusions: The presence of H. pylori in oral cavity is more frequent in seropositive subjects without dyspepsia symptoms and could represent the source of gastric infection and bacterial transmission. The data suggest that more than one H. pylori strain may exist in the mouth of asymptomatic persons. © Medicina Ora.
Original languageAmerican English
JournalMedicina Oral, Patologia Oral y Cirugia Bucal
DOIs
StatePublished - 1 Mar 2011

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Dyspepsia
Helicobacter pylori
Mouth
Genotype
Oral Hygiene
Alleles
Dental Plaque
Polymerase Chain Reaction
Infectious Disease Transmission
Seroepidemiologic Studies
Saliva
Habits
Immunoglobulin M
Stomach
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Antibodies

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Fernández-Tilapa, Gloria ; Axinecuilteco-Hilera, Jazmín ; Giono-Cerezo, Silvia ; Martínez-Carrillo, Dinorah Nashely ; Illades-Aguiar, Berenice ; Román-Román, Adolfo. / vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia. In: Medicina Oral, Patologia Oral y Cirugia Bucal. 2011.
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title = "vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia",
abstract = "Objective: The aims of this research were to determine the prevalence of Helicobacter pylori and its vacA genotypes in oral cavity in persons without dyspepsia and to establish the association between the presence of H. pylori in oral cavity and oral hygiene. The seroprevalence of anti-H. pylori antibodies and its associated factors were analyzed too. Study design: For the study, 200 adults without dyspepsia symptoms were selected. Dental plaque and saliva samples from each subject were obtained. H. pylori detection in oral samples was carried out by polymerase chain reaction (PCR) and for vacA genotyping a semi-nested and nested PCR was used. The enzyme-linked immunosorbent assay (ELISA) was used to detect anti-H. pylori IgG and IgM. The data were analyzed with Chi square and Fisher exact test and the statistical significance was set to 0.05. Results: Of 200 subjects tested, 124 (62{\%}) were seropositive. H. pylori was detected in the oral cavity of 34 subjects (17{\%}) and vacA allelotypes were typified in 12 of those samples. The s1 allele was detected in 8 (66.7{\%}) samples and in one of them m1 and m2 alleles were found. In four subjects vacA m1 subtypes were found and in two of those both m1 and m2 alleles were detected. The prevalence of H. pylori in oral cavity was higher (l8.5{\%}) among seropositive subjects compared with seronegative persons. No association was found between the presence of H. pylori and oral hygiene habits. Conclusions: The presence of H. pylori in oral cavity is more frequent in seropositive subjects without dyspepsia symptoms and could represent the source of gastric infection and bacterial transmission. The data suggest that more than one H. pylori strain may exist in the mouth of asymptomatic persons. {\circledC} Medicina Ora.",
author = "Gloria Fern{\'a}ndez-Tilapa and Jazm{\'i}n Axinecuilteco-Hilera and Silvia Giono-Cerezo and Mart{\'i}nez-Carrillo, {Dinorah Nashely} and Berenice Illades-Aguiar and Adolfo Rom{\'a}n-Rom{\'a}n",
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vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia. / Fernández-Tilapa, Gloria; Axinecuilteco-Hilera, Jazmín; Giono-Cerezo, Silvia; Martínez-Carrillo, Dinorah Nashely; Illades-Aguiar, Berenice; Román-Román, Adolfo.

In: Medicina Oral, Patologia Oral y Cirugia Bucal, 01.03.2011.

Research output: Contribution to journalArticle

TY - JOUR

T1 - vacA genotypes in oral cavity and Helicobacter pylori seropositivity among adults without dyspepsia

AU - Fernández-Tilapa, Gloria

AU - Axinecuilteco-Hilera, Jazmín

AU - Giono-Cerezo, Silvia

AU - Martínez-Carrillo, Dinorah Nashely

AU - Illades-Aguiar, Berenice

AU - Román-Román, Adolfo

PY - 2011/3/1

Y1 - 2011/3/1

N2 - Objective: The aims of this research were to determine the prevalence of Helicobacter pylori and its vacA genotypes in oral cavity in persons without dyspepsia and to establish the association between the presence of H. pylori in oral cavity and oral hygiene. The seroprevalence of anti-H. pylori antibodies and its associated factors were analyzed too. Study design: For the study, 200 adults without dyspepsia symptoms were selected. Dental plaque and saliva samples from each subject were obtained. H. pylori detection in oral samples was carried out by polymerase chain reaction (PCR) and for vacA genotyping a semi-nested and nested PCR was used. The enzyme-linked immunosorbent assay (ELISA) was used to detect anti-H. pylori IgG and IgM. The data were analyzed with Chi square and Fisher exact test and the statistical significance was set to 0.05. Results: Of 200 subjects tested, 124 (62%) were seropositive. H. pylori was detected in the oral cavity of 34 subjects (17%) and vacA allelotypes were typified in 12 of those samples. The s1 allele was detected in 8 (66.7%) samples and in one of them m1 and m2 alleles were found. In four subjects vacA m1 subtypes were found and in two of those both m1 and m2 alleles were detected. The prevalence of H. pylori in oral cavity was higher (l8.5%) among seropositive subjects compared with seronegative persons. No association was found between the presence of H. pylori and oral hygiene habits. Conclusions: The presence of H. pylori in oral cavity is more frequent in seropositive subjects without dyspepsia symptoms and could represent the source of gastric infection and bacterial transmission. The data suggest that more than one H. pylori strain may exist in the mouth of asymptomatic persons. © Medicina Ora.

AB - Objective: The aims of this research were to determine the prevalence of Helicobacter pylori and its vacA genotypes in oral cavity in persons without dyspepsia and to establish the association between the presence of H. pylori in oral cavity and oral hygiene. The seroprevalence of anti-H. pylori antibodies and its associated factors were analyzed too. Study design: For the study, 200 adults without dyspepsia symptoms were selected. Dental plaque and saliva samples from each subject were obtained. H. pylori detection in oral samples was carried out by polymerase chain reaction (PCR) and for vacA genotyping a semi-nested and nested PCR was used. The enzyme-linked immunosorbent assay (ELISA) was used to detect anti-H. pylori IgG and IgM. The data were analyzed with Chi square and Fisher exact test and the statistical significance was set to 0.05. Results: Of 200 subjects tested, 124 (62%) were seropositive. H. pylori was detected in the oral cavity of 34 subjects (17%) and vacA allelotypes were typified in 12 of those samples. The s1 allele was detected in 8 (66.7%) samples and in one of them m1 and m2 alleles were found. In four subjects vacA m1 subtypes were found and in two of those both m1 and m2 alleles were detected. The prevalence of H. pylori in oral cavity was higher (l8.5%) among seropositive subjects compared with seronegative persons. No association was found between the presence of H. pylori and oral hygiene habits. Conclusions: The presence of H. pylori in oral cavity is more frequent in seropositive subjects without dyspepsia symptoms and could represent the source of gastric infection and bacterial transmission. The data suggest that more than one H. pylori strain may exist in the mouth of asymptomatic persons. © Medicina Ora.

U2 - 10.4317/medoral.16.e175

DO - 10.4317/medoral.16.e175

M3 - Article

JO - Medicina Oral, Patologia Oral y Cirugia Bucal

JF - Medicina Oral, Patologia Oral y Cirugia Bucal

SN - 1698-4447

ER -