V2 from a curtovirus is a suppressor of post-transcriptional gene silencing

Ana P. Luna, Edgar A. Rodríguez-Negrete, Gabriel Morilla, Liping Wang, Rosa Lozano-Durán, Araceli G. Castillo, Eduardo R. Bejarano

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The suppression of gene silencing is a key mechanism for the success of viral infection in plants. DNA viruses from the Geminiviridae family encode several proteins that suppress transcriptional and post-transcriptional gene silencing (TGS/ PTGS). In Begomovirus, the most abundant genus of this family, three out of six genome-encoded proteins, namely C2, C4 and V2, have been shown to suppress PTGS, with V2 being the strongest PTGS suppressor in transient assays. Beet curly top virus (BCTV), the model species for the Curtovirus genus, is able to infect the widest range of plants among geminiviruses. In this genus, only one protein, C2/L2, has been described as inhibiting PTGS. We show here that, despite the lack of sequence homology with its begomoviral counterpart, BCTV V2 acts as a potent PTGS suppressor, possibly by impairing the RDR6 (RNA-dependent RNA polymerase 6)/suppressor of gene silencing 3 (SGS3) pathway.

Original languageEnglish
Article number000933
Pages (from-to)2607-2614
Number of pages8
JournalJournal of General Virology
Volume98
Issue number10
DOIs
StatePublished - Oct 2017

Keywords

  • BCTV
  • Geminivirus
  • RDR6
  • RNA silencing
  • Silencing suppressor
  • V2

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