Using molecular techniques applied to beneficial microorganisms as biotechnological tools for controlling agricultural plant pathogens and pest

In this chapter we present an overview of the use of molecular techniques applied to beneficial microorganisms as biotechnological tools for the control of pathogens and pests of agricultural plants. The content of the chapter addresses issues such as: agricultural pests, diversity of pests that affect economically important media: viruses, bacteria, fungi, nematodos, protozoa, mites, and insects. The biological methods are based in use the natural enemies. On the other hand, we the use of the biological control method to reduce pests in agriculture, the study of different natural enemies and attacks against pests such as bacteria, fungi and nematodes. Respect to the molecular techniques chapter also highlights techniques such as: RACE-PCR, RT-PCR, cloning of genes, nested PCR and qPCR or Real-time PCR. The omics products show great efficacy in the detection of the metabolome and the expressome of several biological control agents, such as Trichoderma spp. and other microoganisms.