TY - JOUR
T1 - Usefulness of Chromogenic CromoCen® AGN agar medium for the identification of the genus Aeromonas
T2 - Assessment of faecal samples
AU - Aguilera-Arreola, M. G.
AU - Portillo-Muñoz, M. I.
AU - Rodríguez-Martínez, C.
AU - Castro-Escarpulli, G.
PY - 2012/8
Y1 - 2012/8
N2 - Selective screening media for the detection and identification of Aeromonas strains are needed to guide primary isolation procedures in the clinical laboratory. This study compared the selective CromoCen® AGN chromogenic agar medium for the detection and identification of Aeromonas strains that were isolated from various samples against the conventional selective agar media that are commonly used for the isolation of this organism in food, environmental and clinical samples. The Miles and Misra and ecometric methods were used to evaluate the microbiological performance of CromoCen® AGN chromogenic agar medium, which was shown to be satisfactory. A total of 14 reference Aeromonas strains, 44 wild strains and 106 clinical stool specimens were examined using both non-chromogenic selective agars that are commonly used for Aeromonas isolation and CromoCen® AGN agar. The latter exhibited 94.73% sensitivity and 100% specificity for the various samples. On CromoCen® AGN agar medium, Aeromonas formed colonies with light green, greenish and salmon pigments with or without a surrounding wide transparent zone (halo) of 2-3. mm in diameter around the entire border. This medium is recommended for the isolation and potential identification of the Aeromonas genus.
AB - Selective screening media for the detection and identification of Aeromonas strains are needed to guide primary isolation procedures in the clinical laboratory. This study compared the selective CromoCen® AGN chromogenic agar medium for the detection and identification of Aeromonas strains that were isolated from various samples against the conventional selective agar media that are commonly used for the isolation of this organism in food, environmental and clinical samples. The Miles and Misra and ecometric methods were used to evaluate the microbiological performance of CromoCen® AGN chromogenic agar medium, which was shown to be satisfactory. A total of 14 reference Aeromonas strains, 44 wild strains and 106 clinical stool specimens were examined using both non-chromogenic selective agars that are commonly used for Aeromonas isolation and CromoCen® AGN agar. The latter exhibited 94.73% sensitivity and 100% specificity for the various samples. On CromoCen® AGN agar medium, Aeromonas formed colonies with light green, greenish and salmon pigments with or without a surrounding wide transparent zone (halo) of 2-3. mm in diameter around the entire border. This medium is recommended for the isolation and potential identification of the Aeromonas genus.
KW - Aeromonas isolation
KW - Chromogenic medium
KW - Ecometric technique
UR - http://www.scopus.com/inward/record.url?scp=84861733456&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2012.04.013
DO - 10.1016/j.mimet.2012.04.013
M3 - Artículo
SN - 0167-7012
VL - 90
SP - 100
EP - 104
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -