TY - JOUR
T1 - Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts
AU - Arrieta Baez, Daniel
AU - Zepeda Vallejo, Gerardo
AU - Cano Sánchez, Patricia
AU - Breña Valle, Matilde
AU - Reyes Chilpa, Ricardo
AU - Jiménez Estrada, Manuel
PY - 2002
Y1 - 2002
N2 - An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di-O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity-guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
AB - An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di-O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity-guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
KW - Antitumoral compounds
KW - Flavonoids
KW - Genotoxic compounds
KW - SOS-chromotest
KW - Senna
UR - http://www.scopus.com/inward/record.url?scp=0036181998&partnerID=8YFLogxK
M3 - Artículo
SN - 0261-1929
VL - 30
SP - 87
EP - 92
JO - ATLA Alternatives to Laboratory Animals
JF - ATLA Alternatives to Laboratory Animals
IS - 1
ER -