TY - JOUR
T1 - Use of magnetic polyaniline/maghemite nanocomposite for DNA retrieval from aqueous solutions
AU - Medina-Llamas, Juan Carlos
AU - Chávez-Guajardo, Alicia Elizabeth
AU - Andrade, Cesar Augusto Souza
AU - Alves, Kleber Gonçalves Bezerra
AU - de Melo, Celso Pinto
N1 - Publisher Copyright:
© 2014 Elsevier Inc..
PY - 2014/11/5
Y1 - 2014/11/5
N2 - We demonstrated that the magnetic polyaniline/maghemite nanocomposite (Pani/γ-Fe2O3 MNC) is an efficient agent for retrieval of pure double stranded deoxyribonucleic acid (dsDNA) chains from aqueous solutions. The dsDNA chains used in the retrieval experiments were of sodium salt of Salmon Sperm DNA. Based on λ=260nm absorption measurements, we have employed UV-Vis spectroscopy to estimate the concentration of DNA present in solutions, before and after the interaction with the MNC. The best results corresponded to a maximum amount of 75.2mg of DNA absorbed per gram of MNC reached within only 10min of joint exposure into the aqueous solution. After magnetic separation of the fully DNA-loaded Pani/γ-Fe2O3 MNC, we achieved essentially complete DNA desorption by appropriate changes in the pH of the solution. We have shown that it is possible to recycle the use of these MNC in several adsorption-desorption cycles. By comparing the present results to those of other DNA retrieval systems reported in the literature, we argued that the Pani/γ-Fe2O3 MNC here described represent a promising low-cost material for use as a fast, simple and efficient method of DNA separation and concentration.
AB - We demonstrated that the magnetic polyaniline/maghemite nanocomposite (Pani/γ-Fe2O3 MNC) is an efficient agent for retrieval of pure double stranded deoxyribonucleic acid (dsDNA) chains from aqueous solutions. The dsDNA chains used in the retrieval experiments were of sodium salt of Salmon Sperm DNA. Based on λ=260nm absorption measurements, we have employed UV-Vis spectroscopy to estimate the concentration of DNA present in solutions, before and after the interaction with the MNC. The best results corresponded to a maximum amount of 75.2mg of DNA absorbed per gram of MNC reached within only 10min of joint exposure into the aqueous solution. After magnetic separation of the fully DNA-loaded Pani/γ-Fe2O3 MNC, we achieved essentially complete DNA desorption by appropriate changes in the pH of the solution. We have shown that it is possible to recycle the use of these MNC in several adsorption-desorption cycles. By comparing the present results to those of other DNA retrieval systems reported in the literature, we argued that the Pani/γ-Fe2O3 MNC here described represent a promising low-cost material for use as a fast, simple and efficient method of DNA separation and concentration.
KW - DNA retrieval
KW - Pani/γ-Fe<inf>2</inf>O<inf>3</inf> magnetic nanocomposite
KW - UV-Vis spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=84924083079&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2014.08.002
DO - 10.1016/j.jcis.2014.08.002
M3 - Artículo
C2 - 25203907
SN - 0021-9797
VL - 434
SP - 167
EP - 174
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -