TY - JOUR
T1 - Aprovechamiento de almidón de cebada de mala calidad para la producción de enzimas amilolíticas por aureobasidium pullulans
AU - Hernández-Castillo, María Isabel
AU - Hernández-Navarrete, Angélica
AU - Jiménez-Montejo, Fabiola Eloísa
AU - Cruz-López, María del Carmen
AU - Hidalgo-Lara, María Eugenia
AU - López Y López, Víctor Eric
N1 - Publisher Copyright:
© 2019, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved.
PY - 2019
Y1 - 2019
N2 - Low quality barley that has been rejected for the malt production process can be used to produce value added products, particularly enzymes. Hence, in this work the starch was extracted and used to produce amylolytic enzymes by Aureobasidium pullulans. Using 50 g/L of barley raw starch, glucoamylase, a-amylase, a glucosidase and Β-glucosidase enzymes were produced with maximum activities of 193, 7.4, 8.1 and 8.5 U/mL, respectively. After 30 h of fermentation a crude extract was obtained, which was used as enzymatic source for the saccharification of starch from three sources at 10 g/L: barley, soluble (from potato) and corn. First, two reaction solvents (water and acetate buffer) and the addition of calcium (Ca2+) were evaluated. Afterwards, starch gelatinization was evaluated and finally experiments varying the starch concentration (from 50 to 400 g/L) were conducted. Using water as solvent and the Ca2+ addition had a positive effect, resulting in a maximum glucose concentration of 3.84, 0.84 and 0.4 g/L from barley, soluble and corn starches, respectively. In the other hand, starch gelatinization was positive for soluble and corn starches, enhancing hydrolysis in 23 and 25 %, respectively. Increasing the starch concentration to 50 g/L was the best condition, enhancing hydrolysis in 3.2, 40 and 42 % from barley, soluble and corn starches, respectively. This work demonstrates that low quality raw barley starch can be used for the production of value added products such as enzymes.
AB - Low quality barley that has been rejected for the malt production process can be used to produce value added products, particularly enzymes. Hence, in this work the starch was extracted and used to produce amylolytic enzymes by Aureobasidium pullulans. Using 50 g/L of barley raw starch, glucoamylase, a-amylase, a glucosidase and Β-glucosidase enzymes were produced with maximum activities of 193, 7.4, 8.1 and 8.5 U/mL, respectively. After 30 h of fermentation a crude extract was obtained, which was used as enzymatic source for the saccharification of starch from three sources at 10 g/L: barley, soluble (from potato) and corn. First, two reaction solvents (water and acetate buffer) and the addition of calcium (Ca2+) were evaluated. Afterwards, starch gelatinization was evaluated and finally experiments varying the starch concentration (from 50 to 400 g/L) were conducted. Using water as solvent and the Ca2+ addition had a positive effect, resulting in a maximum glucose concentration of 3.84, 0.84 and 0.4 g/L from barley, soluble and corn starches, respectively. In the other hand, starch gelatinization was positive for soluble and corn starches, enhancing hydrolysis in 23 and 25 %, respectively. Increasing the starch concentration to 50 g/L was the best condition, enhancing hydrolysis in 3.2, 40 and 42 % from barley, soluble and corn starches, respectively. This work demonstrates that low quality raw barley starch can be used for the production of value added products such as enzymes.
KW - A-glucosidase
KW - Glucoamylase
KW - Saccharification
KW - Β-glucosidase
KW - α-amylase
UR - http://www.scopus.com/inward/record.url?scp=85072814238&partnerID=8YFLogxK
U2 - 10.20937/RICA.2019.35.02.14
DO - 10.20937/RICA.2019.35.02.14
M3 - Artículo
AN - SCOPUS:85072814238
SN - 0188-4999
VL - 35
SP - 435
EP - 446
JO - Revista Internacional de Contaminacion Ambiental
JF - Revista Internacional de Contaminacion Ambiental
IS - 2
ER -