Ursolic and oleanolic acids as antimicrobial and immunomodulatory compounds for tuberculosis treatment

Adelina Jiménez-Arellanes, Julieta Luna-Herrera, Jorge Cornejo-Garrido, Sonia López-García, María E. Castro-Mussot, Mariana Meckes-Fischer, Dulce Mata-Espinosa, Brenda Marquina, Javier Torres, Rogelio Hernández-Pando

Research output: Contribution to journalArticlepeer-review

109 Scopus citations

Abstract

Background: New alternatives for the treatment of Tuberculosis (TB) are urgently needed and medicinal plants represent a potential option. Chamaedora tepejilote and Lantana hispida are medicinal plants from Mexico and their hexanic extracts have shown antimycobacterial activity. Bioguided investigation of these extracts showed that the active compounds were ursolic acid (UA) and oleanolic acid (OA). Methods: The activity of UA and OA against Mycobacterium tuberculosis H37Rv, four monoresistant strains, and two drug-resistant clinical isolates were determined by MABA test. The intracellular activity of UA and OA against M. tuberculosis H37Rv and a MDR clinical isolate were evaluated in a macrophage cell line. Finally, the antitubercular activity of UA and OA was tested in BALB/c mice infected with M. tuberculosis H37Rv or a MDR strain, by determining pulmonary bacilli loads, tissue damage by automated histomorphometry, and expression of IFN-γ, TNF-α, and iNOS by quantitative RT-PCR. Results: The in vitro assay showed that the UA/OA mixture has synergistic activity. The intracellular activity of these compounds against M. tuberculosis H37Rv and a MDR clinical isolate in a macrophage cell line showed that both compounds, alone and in combination, were active against intracellular mycobacteria even at low doses. Moreover, when both compounds were used to treat BALB/c mice with TB induced by H37Rv or MDR bacilli, a significant reduction of bacterial loads and pneumonia were observed compared to the control. Interestingly, animals treated with UA and OA showed a higher expression of IFN-γ and TNF-α in their lungs, than control animals. Conclusion: UA and OA showed antimicrobial activity plus an immune-stimulatory effect that permitted the control of experimental pulmonary TB.

Original languageEnglish
Article number258
JournalBMC Complementary and Alternative Medicine
Volume13
DOIs
StatePublished - 7 Oct 2013

Keywords

  • Antimycobacterial activity
  • Antitubercular activity
  • Medicinal plants
  • Triterpenoids

Fingerprint

Dive into the research topics of 'Ursolic and oleanolic acids as antimicrobial and immunomodulatory compounds for tuberculosis treatment'. Together they form a unique fingerprint.

Cite this