@article{07594cc8c9a5450eaa0a7f07f6f4e348,
title = "Twenty-four microsatellite markers for the gray mouse opossum (Tlacuatzin canescens): Development from illumina paired-end sequences",
abstract = "Tlacuatzin canescens is an opossum species endemic to Mexico from southern Sonora to Oaxaca, with populations in Tres Mar{\'i}as Islands and in central Peninsula of Yucatan. A technique based on Illumina paired-end sequencing of a library highly enriched for microsatellite repeats was used to develop loci. Twenty-four polymorphic (tri- and tetranucleotide) microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 8). Levels of expected heterozygosity across all markers was fairly low (mean HE = 0. 477, mean HO = 0. 480).",
keywords = "Illumina, Microsatellites, PAL_FINDER, Tlacuatzin canescens",
author = "J{\'e}sica Arcangeli and Cervantes, {Fernando A.} and Lance, {Stacey L.} and Salazar, {Ma Isabel} and Jorge Ortega",
note = "Funding Information: Acknowledgments Financial support was provided by CONACyT Ciencia B{\'a}sica (156725). Some tissue samples were obtained from field work supported by UNAM through grant PAPIIT (222206-2). J{\'e}sica Arcangeli thanks Posgrado en Ciencias Biol{\'o}gicas, UNAM, and presets this paper as a requirement to obtain Ph. D. degree. Tissue samples/museum specimens were provided by C. L{\'o}pez-Gonz{\'a}lez (CIDIIR-Durango), and C. Lorenzo (ECOSUR-SC-M). Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation. J{\'e}sica Arcangeli is supported by a scholarship provided by CONACyT (23059) as a Ph.D. student in Posgrado en Ciencias Biol{\'o}gicas, UNAM.",
year = "2013",
month = jun,
doi = "10.1007/s12686-012-9805-4",
language = "Ingl{\'e}s",
volume = "5",
pages = "367--370",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
number = "2",
}