TY - JOUR
T1 - Turbidity as a useful optical parameter to predict protein crystallization by dynamic light scattering
AU - Moreno, A.
AU - Mas-Oliva, J.
AU - Soriano-García, M.
AU - Oliver Salvador, C.
AU - Martín Bolaños-García, V.
N1 - Funding Information:
Abel Moreno and J. Mas-Oliva acknowledge financial support from DGAPA-UNAM (Grants IN 218597 and IN 215397, respectively).
PY - 2000/2/29
Y1 - 2000/2/29
N2 - The aggregation behavior of several proteins in solution including the human apolipoproteins A-II and C-III, as well as concanavalin A, thaumatin, lysozyme and mexicain, is discussed based on dynamic light scattering techniques. According to our results, the estimation of parameters such as the geometrical factor (H) and turbidity (τ) under different environmental conditions, is a useful approach in order to elucidate if protein aggregation is carried out by either nucleation or random mechanisms. We conclude that dynamic light scattering, an accurate and non-destructive technique, can be used to determine either protein precrystallization parameters or crystallization conditions when both H and r are taken into account. (C) 2000 Elsevier Science B.V.
AB - The aggregation behavior of several proteins in solution including the human apolipoproteins A-II and C-III, as well as concanavalin A, thaumatin, lysozyme and mexicain, is discussed based on dynamic light scattering techniques. According to our results, the estimation of parameters such as the geometrical factor (H) and turbidity (τ) under different environmental conditions, is a useful approach in order to elucidate if protein aggregation is carried out by either nucleation or random mechanisms. We conclude that dynamic light scattering, an accurate and non-destructive technique, can be used to determine either protein precrystallization parameters or crystallization conditions when both H and r are taken into account. (C) 2000 Elsevier Science B.V.
KW - Dynamic light scattering
KW - Geometrical factor
KW - Human apolipoproteins
KW - Protein crystallization
KW - Turbidity
UR - http://www.scopus.com/inward/record.url?scp=0034728443&partnerID=8YFLogxK
U2 - 10.1016/S0022-2860(99)00318-X
DO - 10.1016/S0022-2860(99)00318-X
M3 - Artículo
SN - 0022-2860
VL - 519
SP - 243
EP - 256
JO - Journal of Molecular Structure
JF - Journal of Molecular Structure
IS - 1-3
ER -