Transient responses of Wickerhamia sp. yeast continuous cultures to qualitative changes in carbon source supply: induction and catabolite repression of α-amylase synthesis

Griselda Ma Chávez-Camarillo, Uriel Mauricio Santiago-Flores, Armando Mena-Vivanco, Liliana Morales-Barrera, Elias Cortés-Acosta, Eliseo Cristiani-Urbina

Research output: Contribution to journalArticle

Abstract

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan. The purpose of this study was to evaluate the inductive effect of starch and maltose, and the repressive/inhibitory effect of glucose, on amy-1 gene expression and α-amylase production by Wickerhamia sp., using continuous culture under transient-state conditions at a dilution rate (D) of 0.083 h−1. Induction and repression kinetics of α-amylase were studied by changing the medium feed from glucose to maltose or starch in the induction experiments and vice versa in the repression experiments. Expression levels of amy-1 gene were measured by RT-qPCR. Results showed that starch was a more efficient inducer of α-amylase synthesis compared to maltose, with maximum accumulation rate constants of 0.424 and 0.191 h−1, respectively. In contrast, α-amylase synthesis in starch and maltose cultures was partially repressed by glucose as indicated by a specific activity close to basal levels and a decay constant rate (− 0.065 and − 0.069 h−1, respectively) higher than − D. A linear dependence of the specific rate of α-amylase production on mRNA relative abundance of amy-1 gene was observed. An inhibitory effect of glucose was not observed even at a concentration of 30 g L−1. In conclusion, the transient continuous culture is a useful tool to determine the qualitative and quantitative effects of maltose and starch on α-amylase induction and of glucose on enzyme repression, as well as to obtain a detailed understanding of the dynamic behavior of the yeast culture. Furthermore, results showed that amylaceous substrates can be very effective carbon sources for the production of α-amylase without being inhibited by glucose.
Original languageAmerican English
Pages (from-to)625-635
Number of pages561
JournalAnnals of Microbiology
DOIs
StatePublished - 1 Oct 2018

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Catabolite Repression
Amylases
Maltose
Carbon
Yeasts
Starch
Glucose
Enzyme Repression
Genes
Germany
Gene Expression
Messenger RNA

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title = "Transient responses of Wickerhamia sp. yeast continuous cultures to qualitative changes in carbon source supply: induction and catabolite repression of α-amylase synthesis",
abstract = "{\circledC} 2018, Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan. The purpose of this study was to evaluate the inductive effect of starch and maltose, and the repressive/inhibitory effect of glucose, on amy-1 gene expression and α-amylase production by Wickerhamia sp., using continuous culture under transient-state conditions at a dilution rate (D) of 0.083 h−1. Induction and repression kinetics of α-amylase were studied by changing the medium feed from glucose to maltose or starch in the induction experiments and vice versa in the repression experiments. Expression levels of amy-1 gene were measured by RT-qPCR. Results showed that starch was a more efficient inducer of α-amylase synthesis compared to maltose, with maximum accumulation rate constants of 0.424 and 0.191 h−1, respectively. In contrast, α-amylase synthesis in starch and maltose cultures was partially repressed by glucose as indicated by a specific activity close to basal levels and a decay constant rate (− 0.065 and − 0.069 h−1, respectively) higher than − D. A linear dependence of the specific rate of α-amylase production on mRNA relative abundance of amy-1 gene was observed. An inhibitory effect of glucose was not observed even at a concentration of 30 g L−1. In conclusion, the transient continuous culture is a useful tool to determine the qualitative and quantitative effects of maltose and starch on α-amylase induction and of glucose on enzyme repression, as well as to obtain a detailed understanding of the dynamic behavior of the yeast culture. Furthermore, results showed that amylaceous substrates can be very effective carbon sources for the production of α-amylase without being inhibited by glucose.",
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Transient responses of Wickerhamia sp. yeast continuous cultures to qualitative changes in carbon source supply: induction and catabolite repression of α-amylase synthesis. / Chávez-Camarillo, Griselda Ma; Santiago-Flores, Uriel Mauricio; Mena-Vivanco, Armando; Morales-Barrera, Liliana; Cortés-Acosta, Elias; Cristiani-Urbina, Eliseo.

In: Annals of Microbiology, 01.10.2018, p. 625-635.

Research output: Contribution to journalArticle

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AU - Chávez-Camarillo, Griselda Ma

AU - Santiago-Flores, Uriel Mauricio

AU - Mena-Vivanco, Armando

AU - Morales-Barrera, Liliana

AU - Cortés-Acosta, Elias

AU - Cristiani-Urbina, Eliseo

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AB - © 2018, Springer-Verlag GmbH Germany, part of Springer Nature and the University of Milan. The purpose of this study was to evaluate the inductive effect of starch and maltose, and the repressive/inhibitory effect of glucose, on amy-1 gene expression and α-amylase production by Wickerhamia sp., using continuous culture under transient-state conditions at a dilution rate (D) of 0.083 h−1. Induction and repression kinetics of α-amylase were studied by changing the medium feed from glucose to maltose or starch in the induction experiments and vice versa in the repression experiments. Expression levels of amy-1 gene were measured by RT-qPCR. Results showed that starch was a more efficient inducer of α-amylase synthesis compared to maltose, with maximum accumulation rate constants of 0.424 and 0.191 h−1, respectively. In contrast, α-amylase synthesis in starch and maltose cultures was partially repressed by glucose as indicated by a specific activity close to basal levels and a decay constant rate (− 0.065 and − 0.069 h−1, respectively) higher than − D. A linear dependence of the specific rate of α-amylase production on mRNA relative abundance of amy-1 gene was observed. An inhibitory effect of glucose was not observed even at a concentration of 30 g L−1. In conclusion, the transient continuous culture is a useful tool to determine the qualitative and quantitative effects of maltose and starch on α-amylase induction and of glucose on enzyme repression, as well as to obtain a detailed understanding of the dynamic behavior of the yeast culture. Furthermore, results showed that amylaceous substrates can be very effective carbon sources for the production of α-amylase without being inhibited by glucose.

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