TY - JOUR
T1 - Transcriptional response of immune-related genes in Litopenaeus vannamei post-larvae cultured in recirculating aquaculture systems with and without biofloc
AU - Soto-Alcalá, Jorge
AU - Álvarez-Ruiz, Píndaro
AU - Audelo-Naranjo, J. M.
AU - Esparza-Leal, H. M.
AU - Luis-Villaseñor, I. E.
AU - Estrada-Godínez, J. A.
AU - Luna-González, A.
AU - Gámez-Jiménez, C.
AU - Diarte-Plata, G.
N1 - Publisher Copyright:
© 2018, Springer Nature Switzerland AG.
PY - 2019/2/15
Y1 - 2019/2/15
N2 - In the present study, quantitative real-time RT-PCR was used to monitor the transcriptional responses of seven key genes related to some innate immune pathways in shrimp postlarvae after being placed in a recirculating aquaculture system (RAS) with or without biofloc (BF or no-BF). Each system consisted of the main tank with 400 L of seawater and six glass aquariums (50 L each). Besides, the nucleotide sequences of myosin light chain (LvMyo) from Litopenaeus vannamei related to the phagocytosis pathway were described. The sequence analysis indicated that LvMyo is a conserved protein among crustaceans and is present in other arthropods. The transcriptional response to the treatments showed several expression patterns. The prophenoloxidase gene was up-regulated in both systems (P < 0.05) and was higher in BF than in no-BF (P < 0.01). Phagocytosis-related genes depicted differential expressions. LvMyo, Ras-associated binding 6, and Ras-related nuclear protein expressions were higher in BF than in no-BF (P < 0.05). Regarding antioxidant genes, glutathione peroxidase was up-regulated only in BF (P < 0.05). Superoxide dismutase expression was lower in BF at 12 h (P < 0.05), but higher at 24 h (P < 0.05). These findings suggest that biofloc modulates the transcription of genes related to the immune response in shrimp as an early response or at the mid-term. Besides, the biological filter in a RAS without biofloc seems to be able to maintain a bacterial population that promotes a lower but similar response to that induced in the biofloc system.
AB - In the present study, quantitative real-time RT-PCR was used to monitor the transcriptional responses of seven key genes related to some innate immune pathways in shrimp postlarvae after being placed in a recirculating aquaculture system (RAS) with or without biofloc (BF or no-BF). Each system consisted of the main tank with 400 L of seawater and six glass aquariums (50 L each). Besides, the nucleotide sequences of myosin light chain (LvMyo) from Litopenaeus vannamei related to the phagocytosis pathway were described. The sequence analysis indicated that LvMyo is a conserved protein among crustaceans and is present in other arthropods. The transcriptional response to the treatments showed several expression patterns. The prophenoloxidase gene was up-regulated in both systems (P < 0.05) and was higher in BF than in no-BF (P < 0.01). Phagocytosis-related genes depicted differential expressions. LvMyo, Ras-associated binding 6, and Ras-related nuclear protein expressions were higher in BF than in no-BF (P < 0.05). Regarding antioxidant genes, glutathione peroxidase was up-regulated only in BF (P < 0.05). Superoxide dismutase expression was lower in BF at 12 h (P < 0.05), but higher at 24 h (P < 0.05). These findings suggest that biofloc modulates the transcription of genes related to the immune response in shrimp as an early response or at the mid-term. Besides, the biological filter in a RAS without biofloc seems to be able to maintain a bacterial population that promotes a lower but similar response to that induced in the biofloc system.
KW - Biofloc
KW - Gene expression
KW - Glutathione peroxidase
KW - Heterotrophic culture
KW - Phagocytosis
KW - Prophenoloxidase
KW - RAS
KW - Shrimp immune system
KW - Superoxide dismutase
UR - http://www.scopus.com/inward/record.url?scp=85056345955&partnerID=8YFLogxK
U2 - 10.1007/s10499-018-0317-4
DO - 10.1007/s10499-018-0317-4
M3 - Artículo
SN - 0967-6120
VL - 27
SP - 209
EP - 225
JO - Aquaculture International
JF - Aquaculture International
IS - 1
ER -