TY - JOUR
T1 - Transcriptional changes associated with chilling tolerance and susceptibility in 'Micro-Tom' tomato fruit using RNA-Seq
AU - Cruz-Mendívil, Abraham
AU - López-Valenzuela, José A.
AU - Calderón-Vázquez, Carlos L.
AU - Vega-García, Misael O.
AU - Reyes-Moreno, Cuauhtémoc
AU - Valdez-Ortiz, Angel
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Tomato fruit are horticultural products of great economic and nutritional value, whose transportation and marketing at low temperature are limited due to their susceptibility to develop chilling injury (CI). Hot water (HW) pre-treatments have been shown to reduce the CI symptoms in tomato fruit, but the molecular mechanisms involved in the acquisition of CI tolerance remain unclear. In the present work, a comparative transcriptomic analysis between HW treated and non-treated fruit before and after cold storage was carried out. RNA-Seq analysis detected a large number of differentially expressed genes that ranged from 2235 (heat shock) to 5433 (cold storage). Three clusters of genes were identified after 2 weeks of cold storage: the chilling-response included the down-regulation of genes involved in photosynthesis, metabolism of cell wall, lipid and ethylene, as well as the up-regulation of genes for trehalose synthesis and transcription factors (DOF and MYB); the chilling-susceptibility was associated with the down-regulation of genes involved in carotenoid biosynthesis, which correlates with the main CI symptom of uneven ripening; meanwhile, the chilling-tolerance was related to the up-regulation of genes for heat stress (heat shock proteins and heat shock transcription factors) and detoxification (glutathione S-transferases). The induced tolerance to CI in tomato fruit seems to be related first with the protection of cell wall and membranes integrity, and second with the restoration of ethylene biosynthesis and signaling.
AB - Tomato fruit are horticultural products of great economic and nutritional value, whose transportation and marketing at low temperature are limited due to their susceptibility to develop chilling injury (CI). Hot water (HW) pre-treatments have been shown to reduce the CI symptoms in tomato fruit, but the molecular mechanisms involved in the acquisition of CI tolerance remain unclear. In the present work, a comparative transcriptomic analysis between HW treated and non-treated fruit before and after cold storage was carried out. RNA-Seq analysis detected a large number of differentially expressed genes that ranged from 2235 (heat shock) to 5433 (cold storage). Three clusters of genes were identified after 2 weeks of cold storage: the chilling-response included the down-regulation of genes involved in photosynthesis, metabolism of cell wall, lipid and ethylene, as well as the up-regulation of genes for trehalose synthesis and transcription factors (DOF and MYB); the chilling-susceptibility was associated with the down-regulation of genes involved in carotenoid biosynthesis, which correlates with the main CI symptom of uneven ripening; meanwhile, the chilling-tolerance was related to the up-regulation of genes for heat stress (heat shock proteins and heat shock transcription factors) and detoxification (glutathione S-transferases). The induced tolerance to CI in tomato fruit seems to be related first with the protection of cell wall and membranes integrity, and second with the restoration of ethylene biosynthesis and signaling.
KW - Chilling injury
KW - Heat shock
KW - Induced tolerance
KW - Postharvest
KW - Solanum lycopersicum
KW - Transcriptomic profiling
UR - http://www.scopus.com/inward/record.url?scp=84908032595&partnerID=8YFLogxK
U2 - 10.1016/j.postharvbio.2014.08.009
DO - 10.1016/j.postharvbio.2014.08.009
M3 - Artículo
SN - 0925-5214
VL - 99
SP - 141
EP - 151
JO - Postharvest Biology and Technology
JF - Postharvest Biology and Technology
ER -