TY - JOUR
T1 - Toxicological hazard induced by sucralose to environmentally relevant concentrations in common carp (Cyprinus carpio)
AU - Saucedo-Vence, Karinne
AU - Elizalde-Velázquez, Armando
AU - Dublán-García, Octavio
AU - Galar-Martínez, Marcela
AU - Islas-Flores, Hariz
AU - SanJuan-Reyes, Nely
AU - García-Medina, Sandra
AU - Hernández-Navarro, María Dolores
AU - Gómez-Oliván, Leobardo Manuel
N1 - Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Sucralose (SUC) is an artificial sweetener that is now widely used in North American and Europe; it has been detected in a wide variety of aquatic environments. It is considered safe for human consumption but its effects in the ecosystem have not yet been studied in depth, since limited ecotoxicological data are available in the peer-reviewed literature. This study aimed to evaluate potential SUC-induced toxicological hazard in the blood, brain, gill, liver and muscle of Cyprinus carpio using oxidative stress biomarkers. Carps were exposed to two different environmentally relevant concentrations (0.05 and 155 μg L− 1) for different exposure times (12, 24, 48, 72 and 96 h). The following biomarkers were evaluated: lipid peroxidation (LPX), hydroperoxide content (HPC) and protein carbonyl content (PCC), as well as the activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). SUC was determined by high pressure liquid chromatography tandem mass spectrometry techniques (HPLC)–MS/MS. Results show a statically significant increase in LPX, HPC, PCC (P < 0.05) especially in gill, brain and muscle, as well as significant changes in the activity of antioxidant enzymes in gill and muscle. Furthermore, the biomarkers employed in this study are useful in the assessment of the environmental impact of this agent on aquatic species.
AB - Sucralose (SUC) is an artificial sweetener that is now widely used in North American and Europe; it has been detected in a wide variety of aquatic environments. It is considered safe for human consumption but its effects in the ecosystem have not yet been studied in depth, since limited ecotoxicological data are available in the peer-reviewed literature. This study aimed to evaluate potential SUC-induced toxicological hazard in the blood, brain, gill, liver and muscle of Cyprinus carpio using oxidative stress biomarkers. Carps were exposed to two different environmentally relevant concentrations (0.05 and 155 μg L− 1) for different exposure times (12, 24, 48, 72 and 96 h). The following biomarkers were evaluated: lipid peroxidation (LPX), hydroperoxide content (HPC) and protein carbonyl content (PCC), as well as the activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). SUC was determined by high pressure liquid chromatography tandem mass spectrometry techniques (HPLC)–MS/MS. Results show a statically significant increase in LPX, HPC, PCC (P < 0.05) especially in gill, brain and muscle, as well as significant changes in the activity of antioxidant enzymes in gill and muscle. Furthermore, the biomarkers employed in this study are useful in the assessment of the environmental impact of this agent on aquatic species.
KW - Bioconcentration
KW - Cyprinus carpio
KW - LC-MS/MS
KW - Oxidative stress
KW - Sucralose
UR - http://www.scopus.com/inward/record.url?scp=84991584958&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2016.09.230
DO - 10.1016/j.scitotenv.2016.09.230
M3 - Artículo
C2 - 27744200
SN - 0048-9697
VL - 575
SP - 347
EP - 357
JO - Science of the Total Environment
JF - Science of the Total Environment
ER -