Tomato infectious chlorosis virus Associated with Tomato Diseases in Baja California, Mexico

J. Méndez-Lozano, M. A. Magallanes-Tapia, J. L. Romero-Romero, E. Camacho-Beltrán, W. L. Orduño Vega, N. E. Leyva-López, M. E. Santos-Cervantes, R. Félix-Gastélum

Research output: Contribution to journalArticlepeer-review

Abstract

Tomato (Solanum lycopersicum L.) is an important vegetable crop in Mexico. The national production in 2009 was 2,043,814 metric tons with a value of $163,560,636 US. Since 2007, abnormal yellow and crispy leaves were observed in commercial tomato fields in Ensenada County, Baja California, Mexico. In affected fields from two localities (San Quintín Valley and Ensenada), symptomatic plants were randomly distributed and symptoms resembled previous descriptions of crinivirus infections in tomato (3). The symptoms and the presence of whiteflies (Bemisia tabaci and Trialeurodes vaporariorum) in the affected fields suggested a viral etiology. Leaf samples of 143 symptomatic tomato plants were collected in the 2007 and 2008 growing seasons. Total RNA was extracted and analyzed by reverse transcription (RT)-PCR assay for simultaneous detection of Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV). Degenerate primers (HS-11/HS-12) were used in combination with specific primers (TIC-3/TIC-4 ...
Original languageAmerican English
Pages (from-to)1229-1229
Number of pages1
JournalPlant Disease
Volume96
Issue number8
DOIs
StatePublished - Aug 2012

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