Tolerance of a sulfidogenic sludge to trichloroethylene at microcosms level as a basis for a long-term operation of reactors designed for its biodegradation

Mario Alberto Santana-Santos, Alberto Ordaz, Janet Jan-Roblero, Fernando Bastida González, Paola B. Zárate Segura, Claudia Guerrero-Barajas

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Abstract

© 2019, © 2019 Taylor & Francis Group, LLC. Trichloroethylene (TCE) is known as a toxic organic compound found as a pollutant in water streams around the world. The ultimate goal of the present work was to determine the TCE concentration that would be feasible to biodegrade on a long-term basis by a sulfidogenic sludge while maintaining sulfate reducing activity (SRA). Microcosms were prepared with sulfidogenic sludge obtained from a stabilized sulfidogenic UASB and amended with different TCE concentrations (100–300 µM) and two different proportions of volatile fatty acids (VFA) acetate, propionate and butyrate at COD of 2.5:1:1 and 1:1:1, respectively to evaluate the tolerance of the sludge. The overall results suggested that the continuous exposure of the microorganisms to TCE leads to inhibition of SRA; nonetheless, the SRA can be recovered after adequate supplementation of carbon sources and sulfate. The most suitable TCE concentration to operate on a long-term basis while preserving SRA was 26–35 mg L–1 (200–260 µM). A low level of expression of the mRNA of the sulfite reductase subunit alpha (dsrA) gene was obtained in the presence of the TCE and its intermediate products. This gene was associated to SRB belonging to the genera Desulfovibrio, Desulfosalsimonas, Desulfotomaculum, Desulfococcus, Desulfatiglans and Desulfomonas.

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Trichloroethylene
Biodegradation
Genes
Volatile fatty acids
Organic compounds
Microorganisms
Sulfates
Carbon
Water

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title = "Tolerance of a sulfidogenic sludge to trichloroethylene at microcosms level as a basis for a long-term operation of reactors designed for its biodegradation",
abstract = "{\circledC} 2019, {\circledC} 2019 Taylor & Francis Group, LLC. Trichloroethylene (TCE) is known as a toxic organic compound found as a pollutant in water streams around the world. The ultimate goal of the present work was to determine the TCE concentration that would be feasible to biodegrade on a long-term basis by a sulfidogenic sludge while maintaining sulfate reducing activity (SRA). Microcosms were prepared with sulfidogenic sludge obtained from a stabilized sulfidogenic UASB and amended with different TCE concentrations (100–300 µM) and two different proportions of volatile fatty acids (VFA) acetate, propionate and butyrate at COD of 2.5:1:1 and 1:1:1, respectively to evaluate the tolerance of the sludge. The overall results suggested that the continuous exposure of the microorganisms to TCE leads to inhibition of SRA; nonetheless, the SRA can be recovered after adequate supplementation of carbon sources and sulfate. The most suitable TCE concentration to operate on a long-term basis while preserving SRA was 26–35 mg L–1 (200–260 µM). A low level of expression of the mRNA of the sulfite reductase subunit alpha (dsrA) gene was obtained in the presence of the TCE and its intermediate products. This gene was associated to SRB belonging to the genera Desulfovibrio, Desulfosalsimonas, Desulfotomaculum, Desulfococcus, Desulfatiglans and Desulfomonas.",
author = "Santana-Santos, {Mario Alberto} and Alberto Ordaz and Janet Jan-Roblero and {Bastida Gonz{\'a}lez}, Fernando and {Z{\'a}rate Segura}, {Paola B.} and Claudia Guerrero-Barajas",
year = "2019",
month = "1",
day = "1",
doi = "10.1080/10934529.2019.1567157",
language = "American English",
journal = "Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering",
issn = "1093-4529",
publisher = "Taylor and Francis Ltd.",

}

TY - JOUR

T1 - Tolerance of a sulfidogenic sludge to trichloroethylene at microcosms level as a basis for a long-term operation of reactors designed for its biodegradation

AU - Santana-Santos, Mario Alberto

AU - Ordaz, Alberto

AU - Jan-Roblero, Janet

AU - Bastida González, Fernando

AU - Zárate Segura, Paola B.

AU - Guerrero-Barajas, Claudia

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2019, © 2019 Taylor & Francis Group, LLC. Trichloroethylene (TCE) is known as a toxic organic compound found as a pollutant in water streams around the world. The ultimate goal of the present work was to determine the TCE concentration that would be feasible to biodegrade on a long-term basis by a sulfidogenic sludge while maintaining sulfate reducing activity (SRA). Microcosms were prepared with sulfidogenic sludge obtained from a stabilized sulfidogenic UASB and amended with different TCE concentrations (100–300 µM) and two different proportions of volatile fatty acids (VFA) acetate, propionate and butyrate at COD of 2.5:1:1 and 1:1:1, respectively to evaluate the tolerance of the sludge. The overall results suggested that the continuous exposure of the microorganisms to TCE leads to inhibition of SRA; nonetheless, the SRA can be recovered after adequate supplementation of carbon sources and sulfate. The most suitable TCE concentration to operate on a long-term basis while preserving SRA was 26–35 mg L–1 (200–260 µM). A low level of expression of the mRNA of the sulfite reductase subunit alpha (dsrA) gene was obtained in the presence of the TCE and its intermediate products. This gene was associated to SRB belonging to the genera Desulfovibrio, Desulfosalsimonas, Desulfotomaculum, Desulfococcus, Desulfatiglans and Desulfomonas.

AB - © 2019, © 2019 Taylor & Francis Group, LLC. Trichloroethylene (TCE) is known as a toxic organic compound found as a pollutant in water streams around the world. The ultimate goal of the present work was to determine the TCE concentration that would be feasible to biodegrade on a long-term basis by a sulfidogenic sludge while maintaining sulfate reducing activity (SRA). Microcosms were prepared with sulfidogenic sludge obtained from a stabilized sulfidogenic UASB and amended with different TCE concentrations (100–300 µM) and two different proportions of volatile fatty acids (VFA) acetate, propionate and butyrate at COD of 2.5:1:1 and 1:1:1, respectively to evaluate the tolerance of the sludge. The overall results suggested that the continuous exposure of the microorganisms to TCE leads to inhibition of SRA; nonetheless, the SRA can be recovered after adequate supplementation of carbon sources and sulfate. The most suitable TCE concentration to operate on a long-term basis while preserving SRA was 26–35 mg L–1 (200–260 µM). A low level of expression of the mRNA of the sulfite reductase subunit alpha (dsrA) gene was obtained in the presence of the TCE and its intermediate products. This gene was associated to SRB belonging to the genera Desulfovibrio, Desulfosalsimonas, Desulfotomaculum, Desulfococcus, Desulfatiglans and Desulfomonas.

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