Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system

Verónica Zaga-Clavellina, Pilar Flores-Espinosa, Montzerrat Pineda-Torres, Irma Sosa-González, Rodrigo Vega-Sánchez, Guadalupe Estrada-Gutierrez, Aurora Espejel-Núñez, Arturo Flores-Pliego, Rolando Maida-Claros, Higinio Estrada-Juárez, Angel Chávez-Mendoza

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective: Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae. Methods: Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10 6 CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both. Results: Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration. Conclusions: Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region. © 2014 Informa UK Ltd.
Original languageAmerican English
Pages (from-to)1320-1327
Number of pages1187
JournalJournal of Maternal-Fetal and Neonatal Medicine
DOIs
StatePublished - 1 Jan 2014

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    Zaga-Clavellina, V., Flores-Espinosa, P., Pineda-Torres, M., Sosa-González, I., Vega-Sánchez, R., Estrada-Gutierrez, G., Espejel-Núñez, A., Flores-Pliego, A., Maida-Claros, R., Estrada-Juárez, H., & Chávez-Mendoza, A. (2014). Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system. Journal of Maternal-Fetal and Neonatal Medicine, 1320-1327. https://doi.org/10.3109/14767058.2013.857397