TY - JOUR
T1 - Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system
AU - Zaga-Clavellina, Verónica
AU - Flores-Espinosa, Pilar
AU - Pineda-Torres, Montzerrat
AU - Sosa-González, Irma
AU - Vega-Sánchez, Rodrigo
AU - Estrada-Gutierrez, Guadalupe
AU - Espejel-Núñez, Aurora
AU - Flores-Pliego, Arturo
AU - Maida-Claros, Rolando
AU - Estrada-Juárez, Higinio
AU - Chávez-Mendoza, Angel
N1 - Funding Information:
These studies were supported by the Instituto Nacional de Perinatologia ‘‘Isidro Espinosa de los Reyes’’, projects # 06151 and 06161 to VZC. The authors report no conflict of interest.
PY - 2014/9
Y1 - 2014/9
N2 - Objective: Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae. Methods: Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10 6 CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both. Results: Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration. Conclusions: Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region.
AB - Objective: Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae. Methods: Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10 6 CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both. Results: Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration. Conclusions: Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region.
KW - Chorioamnionitis
KW - Human fetal membranes
KW - IL-10
KW - Intrauterine infection
KW - Preterm labor
UR - http://www.scopus.com/inward/record.url?scp=84905455019&partnerID=8YFLogxK
U2 - 10.3109/14767058.2013.857397
DO - 10.3109/14767058.2013.857397
M3 - Artículo
SN - 1476-7058
VL - 27
SP - 1320
EP - 1327
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
IS - 13
ER -