TY - JOUR
T1 - Tissue-specific IL-10 secretion profile from term human fetal membranes stimulated with pathogenic microorganisms associated with preterm labor in a two-compartment tissue culture system
AU - Zaga-Clavellina, Verónica
AU - Flores-Espinosa, Pilar
AU - Pineda-Torres, Montzerrat
AU - Sosa-González, Irma
AU - Vega-Sánchez, Rodrigo
AU - Estrada-Gutierrez, Guadalupe
AU - Espejel-Núñez, Aurora
AU - Flores-Pliego, Arturo
AU - Maida-Claros, Rolando
AU - Estrada-Juárez, Higinio
AU - Chávez-Mendoza, Angel
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Objective: Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae. Methods: Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10 6 CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both. Results: Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration. Conclusions: Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region. © 2014 Informa UK Ltd.
AB - Objective: Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects of pro-inflammatory modulators. The aim of this work was to characterize the contribution of amnion and choriodecidua regions of the human fetal membranes in the production of IL-10 after selective stimulation with Candida albicans, Gardnerella vaginalis and Streptococcus agalactiae. Methods: Pre-labor human fetal membranes were cultured in a two-compartment tissue culture system and stimulated with 1 × 10 6 CFU/ml of each pathogen added to either the amniotic or choriodecidual region or both. Results: Candida albicans and G. vaginalis were the pathogens most effective in inducing IL-10 secretion, increasing 20 and 10 times, respectively, the levels of this cytokine in the choriodecidual compartment. Stimulation with S. agalactiae was effective only in the choriodecidual region, increasing two times IL-10 concentration. Conclusions: Synthesis and secretion of IL-10 in response to three different pathogens associated with intrauterine infection and preterm birth are differential and depend on the nature of the microorganism and initial contact region. © 2014 Informa UK Ltd.
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U2 - 10.3109/14767058.2013.857397
DO - 10.3109/14767058.2013.857397
M3 - Article
SP - 1320
EP - 1327
JO - Journal of Maternal-Fetal and Neonatal Medicine
JF - Journal of Maternal-Fetal and Neonatal Medicine
SN - 1476-7058
ER -