Time-related changes in constitutive and inducible nitric oxide synthases in the rat striatum in a model of Huntington's disease

Penélope Aguilera, María Elena Chánez-Cárdenas, Esaú Floriano-Sánchez, Diana Barrera, Abel Santamaría, Dolores Javier Sánchez-González, Francisca Pérez-Severiano, José Pedraza-Chaverrí, Perla Deyanira Maldonado Jiménez

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Abstract

Excitotoxicity and oxidative stress are mechanisms involved in the neuronal cell death induced by the intrastriatal injection of quinolinic acid (QUIN) as a model of Huntington's disease. Production of nitric oxide by nitric oxide synthase (NOS) has been proposed to participate in QUIN-induced neurotoxicity; however, the precise role of NOS in QUIN-induced toxicity still remains controversial. In order to provide further information on the role of NOS isoforms in QUIN toxicity, we performed real time RT-PCR and immunohistochemistry of inducible NOS (iNOS), endothelial NOS (eNOS) and neuronal NOS (nNOS) and determined Ca2+-dependent and Ca2+-independent NOS activity in a temporal course (3-48 h), after an intrastriatal injection of QUIN to rats. NOS isoforms exhibited a transitory expression of mRNA and protein after QUIN infusion: eNOS increased between 3 and 24 h, iNOS between 12 and 24 h, while nNOS at 35 and 48 h. Ca2+-independent activity (iNOS) did not show any change, while Ca2+-dependent activity (constitutive NOS: eNOS/nNOS) exhibited increased levels at 3 h. Our results support the participation of Ca2+-dependent NOS isoforms during the toxic events produced at early times after QUIN injection. © 2007 Elsevier Inc. All rights reserved.
Original languageAmerican English
Pages (from-to)1200-1207
Number of pages1079
JournalNeuroToxicology
DOIs
StatePublished - 1 Nov 2007
Externally publishedYes

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Aguilera, P., Chánez-Cárdenas, M. E., Floriano-Sánchez, E., Barrera, D., Santamaría, A., Sánchez-González, D. J., Pérez-Severiano, F., Pedraza-Chaverrí, J., & Maldonado Jiménez, P. D. (2007). Time-related changes in constitutive and inducible nitric oxide synthases in the rat striatum in a model of Huntington's disease. NeuroToxicology, 1200-1207. https://doi.org/10.1016/j.neuro.2007.07.010