Thermal Images of Seeds Obtained at Different Depths by Photoacoustic Microscopy (PAM)

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Abstract

The objective of the present study was to obtain thermal images of a broccoli seed (Brassica oleracea) by photoacoustic microscopy, at different modulation frequencies of the incident light beam ((0.5, 1, 5, and 20) Hz). The thermal images obtained in the amplitude of the photoacoustic signal vary with each applied frequency. In the lowest light frequency modulation, there is greater thermal wave penetration in the sample. Likewise, the photoacoustic signal is modified according to the structural characteristics of the sample and the modulation frequency of the incident light. Different structural components could be seen by photothermal techniques, as shown in the present study.

Original languageEnglish
Pages (from-to)812-818
Number of pages7
JournalInternational Journal of Thermophysics
Volume36
Issue number5-6
DOIs
StatePublished - 22 Jun 2015

Keywords

  • Modulation frequency
  • Photoacoustic microscopy
  • Thermal images

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