© 2019, Springer Science+Business Media, LLC, part of Springer Nature. In the present study, the thermal effusivities of human fluids, such as human blood serum, urine, saliva and tears, were obtained by the photopyroelectric (PPE) technique in inverse configuration (IPPE). Human blood serum and urine were obtained from studies carried out in a government hospital for public servants in Mexico City, whereas saliva and tears were artificial. Making use of both, the amplitude and phase of the PPE signal, the thermal effusivities of each one of the mentioned human fluids were obtained. In order to obtain them, the experimental PPE data were normalized to the PPE signal with air as sample and the theoretical PPE equation was fitted to the normalized experimental data.