TY - GEN
T1 - Theragnosis with PDT and IgM antibody anti isoform 4 SOD mitochondrial labeled with PpIX in SiHa, MDA-MB-231 and CaCo-2 cell lines
AU - García, Claudia Martínez
AU - Flores, Yolanda Medina
AU - Vázquez, José Manuel De La Rosa
AU - Soriano-Pérez, Eva Elda
AU - Hernández, Juan Ramón Villalobos
AU - Ramón-Gallegos, Eva
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/10
Y1 - 2019/4/10
N2 - The theragnosis combines functions of diagnosis, treatment and monitoring in real time through images, all with one pharmaceutical agent. IgM Antibody Anti Isoform 4 SOD Mitochondrial labeled with PpIX showed theragnosis activity in lung cancer cell line A-549 in previous work. The main objective of this work was evaluated the theragnostic activity of the IgM Ab in SiHa, MDA-MB-231, CaCo-2 cancer cells and HaCaT non-cancerous cells. The IgM Ab with PpIX was used in different dilutions and irradiated with a LEDs system at 630nm/30J/cm 2 . The immunocytochemistry (ICCs) with the IgM-PpIX Ab in SiHa, MDA-MB-231 and CaCo-2 cell lines presented positive reaction without significant difference, instead, HaCaT cell line presented significant difference (p <0.05). The PDT in the MDA-MB-231 and CaCo-2 cell lines with the IgM-PpIX conjugate decreases its viability up to 76 and 79% in relation to the viability control respectively. Thus IgM-PpIX Ab showed potential in theragnostic in cancerous cell lines MDA-MB-231 and CaCo-2.
AB - The theragnosis combines functions of diagnosis, treatment and monitoring in real time through images, all with one pharmaceutical agent. IgM Antibody Anti Isoform 4 SOD Mitochondrial labeled with PpIX showed theragnosis activity in lung cancer cell line A-549 in previous work. The main objective of this work was evaluated the theragnostic activity of the IgM Ab in SiHa, MDA-MB-231, CaCo-2 cancer cells and HaCaT non-cancerous cells. The IgM Ab with PpIX was used in different dilutions and irradiated with a LEDs system at 630nm/30J/cm 2 . The immunocytochemistry (ICCs) with the IgM-PpIX Ab in SiHa, MDA-MB-231 and CaCo-2 cell lines presented positive reaction without significant difference, instead, HaCaT cell line presented significant difference (p <0.05). The PDT in the MDA-MB-231 and CaCo-2 cell lines with the IgM-PpIX conjugate decreases its viability up to 76 and 79% in relation to the viability control respectively. Thus IgM-PpIX Ab showed potential in theragnostic in cancerous cell lines MDA-MB-231 and CaCo-2.
UR - http://www.scopus.com/inward/record.url?scp=85064872224&partnerID=8YFLogxK
U2 - 10.1063/1.5095908
DO - 10.1063/1.5095908
M3 - Contribución a la conferencia
AN - SCOPUS:85064872224
T3 - AIP Conference Proceedings
BT - XV Mexican Symposium on Medical Physics
A2 - Trevino-Palacios, Carlos-Gerardo
A2 - Garcia-Pelagio, Karla-Paola
A2 - Avila-Aguirre, Olga-Leticia
A2 - Fossion, Ruben
A2 - Brandan, Maria-Ester
A2 - Hernandez-Bojorquez, Mariana
PB - American Institute of Physics Inc.
T2 - 15th Mexican Symposium on Medical Physics
Y2 - 13 June 2018 through 15 June 2018
ER -
