The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable β-1,4-xylanase with exo- and endo-activity

Yolanda García-Huante, Maribel Cayetano-Cruz, Alejandro Santiago-Hernández, Claudia Cano-Ramírez, Rodolfo Marsch-Moreno, Jorge E. Campos, Guillermo Aguilar-Osorio, Claudia G. Benitez-Cardoza, Sergio Trejo-Estrada, María Eugenia Hidalgo-Lara

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

A hyperthermophilic and thermostable xylanase of 82 kDa (TtXynA) was purified from the culture supernatant of T. terrestris Co3Bag1, grown on carboxymethyl cellulose (CMC), and characterized biochemically. TtXynA showed optimal xylanolytic activity at pH 5.5 and at 85 °C, and retained more than 90% of its activity at a broad pH range (4.5–10). The enzyme is highly thermostable with a half-life of 23.1 days at 65 °C, and active in the presence of several metal ions. Circular dichroism spectra strongly suggest the enzyme gains secondary structures when temperature increases. TtXynA displayed higher substrate affinity and higher catalytic efficiency towards beechwood xylan than towards birchwood xylan, oat-spelt xylan, and CMC. According to its final hydrolysis products, TtXynA displays endo-/exo-activity, yielded xylobiose, an unknown oligosaccharide containing about five residues of xylose and a small amount of xylose on beechwood xylan. Finally, this report represents the description of the first fungal hyperthermophilic xylanase which is produced by T. terrestris Co3Bag1. Since TtXynA displays relevant biochemical properties, it may be a suitable candidate for biotechnological applications carried out at high temperatures, like the enzymatic pretreatment of plant biomass for the production of bioethanol.

Original languageEnglish
Pages (from-to)175-186
Number of pages12
JournalExtremophiles
Volume21
Issue number1
DOIs
StatePublished - 1 Jan 2017

Keywords

  • Circular dichroism
  • Endo-xylanase/exo-activity
  • Hyperthermophilic xylanase
  • Thermophilic fungus
  • Thermostable xylanase
  • Thielavia terrestris

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