The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: Comparison of promoter elements and of neighbouring upstream genes

Jorge A. Gonzalez-y-Merchand, M. Joseph Colston, Robert A. Cox

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Abstract

Mycobacterium smegmatis has two rRNA (rrn) operons designated rrnA(f) and rrnB(f). Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 165 rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5'-end of the Box A(L) antitermination element of the leader region of the rrnA(f) operon. The 5'-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single rrn (rrnA(s),) operons of Mycobacterium tuberculosis and Mycobacterium leprae. Primer-extension analysis of the RNA fraction of M. smegmatis revealed four products which were related to transcription start points; the rrnB(f) operon appears to have a single promoter whereas the rrnA(f), operon has three (P1, P2 and P3). Analysis of M. tuberculosis RNA revealed two products corresponding to transcripts directed by promoters homologous with p1 and P3 of the rrnA(f) of M. smegmatis. Thus, the promoter and upstream regions of the rrnA(f) operon of M. smegmatis and the rrnA(s) operon of M. tuberculosis are homologous. The presence of P2 in M. smegmatis and its absence from M. tuberculosis is attributable to insertions/deletions of 97 bp.
Original languageAmerican English
Pages (from-to)667-674
Number of pages8
JournalMicrobiology
DOIs
StatePublished - 1 Jan 1996

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rRNA Operon
Mycobacterium smegmatis
Operon
Mycobacterium tuberculosis
Genes
UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Open Reading Frames
Uridine Diphosphate N-Acetylglucosamine
RNA
Mycobacterium leprae
Transferases
rRNA Genes
Genetic Promoter Regions
Codon
Cell Wall
DNA
Proteins

Cite this

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title = "The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: Comparison of promoter elements and of neighbouring upstream genes",
abstract = "Mycobacterium smegmatis has two rRNA (rrn) operons designated rrnA(f) and rrnB(f). Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 165 rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5'-end of the Box A(L) antitermination element of the leader region of the rrnA(f) operon. The 5'-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single rrn (rrnA(s),) operons of Mycobacterium tuberculosis and Mycobacterium leprae. Primer-extension analysis of the RNA fraction of M. smegmatis revealed four products which were related to transcription start points; the rrnB(f) operon appears to have a single promoter whereas the rrnA(f), operon has three (P1, P2 and P3). Analysis of M. tuberculosis RNA revealed two products corresponding to transcripts directed by promoters homologous with p1 and P3 of the rrnA(f) of M. smegmatis. Thus, the promoter and upstream regions of the rrnA(f) operon of M. smegmatis and the rrnA(s) operon of M. tuberculosis are homologous. The presence of P2 in M. smegmatis and its absence from M. tuberculosis is attributable to insertions/deletions of 97 bp.",
author = "Gonzalez-y-Merchand, {Jorge A.} and Colston, {M. Joseph} and Cox, {Robert A.}",
year = "1996",
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T1 - The rRNA operons of Mycobacterium smegmatis and Mycobacterium tuberculosis: Comparison of promoter elements and of neighbouring upstream genes

AU - Gonzalez-y-Merchand, Jorge A.

AU - Colston, M. Joseph

AU - Cox, Robert A.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Mycobacterium smegmatis has two rRNA (rrn) operons designated rrnA(f) and rrnB(f). Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 165 rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5'-end of the Box A(L) antitermination element of the leader region of the rrnA(f) operon. The 5'-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single rrn (rrnA(s),) operons of Mycobacterium tuberculosis and Mycobacterium leprae. Primer-extension analysis of the RNA fraction of M. smegmatis revealed four products which were related to transcription start points; the rrnB(f) operon appears to have a single promoter whereas the rrnA(f), operon has three (P1, P2 and P3). Analysis of M. tuberculosis RNA revealed two products corresponding to transcripts directed by promoters homologous with p1 and P3 of the rrnA(f) of M. smegmatis. Thus, the promoter and upstream regions of the rrnA(f) operon of M. smegmatis and the rrnA(s) operon of M. tuberculosis are homologous. The presence of P2 in M. smegmatis and its absence from M. tuberculosis is attributable to insertions/deletions of 97 bp.

AB - Mycobacterium smegmatis has two rRNA (rrn) operons designated rrnA(f) and rrnB(f). Appropriate restriction fragments of genomic DNA containing sequences immediately upstream from the 165 rRNA genes were cloned. We now report the nucleotide sequence of 552 bp upstream from the 5'-end of the Box A(L) antitermination element of the leader region of the rrnA(f) operon. The 5'-end of this segment of DNA was found to comprise 113 codons of an ORF encoding a protein which is significantly similar to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (EC 2.5.1.7), which is important to cell wall synthesis. A homologous ORF is located immediately upstream from the single rrn (rrnA(s),) operons of Mycobacterium tuberculosis and Mycobacterium leprae. Primer-extension analysis of the RNA fraction of M. smegmatis revealed four products which were related to transcription start points; the rrnB(f) operon appears to have a single promoter whereas the rrnA(f), operon has three (P1, P2 and P3). Analysis of M. tuberculosis RNA revealed two products corresponding to transcripts directed by promoters homologous with p1 and P3 of the rrnA(f) of M. smegmatis. Thus, the promoter and upstream regions of the rrnA(f) operon of M. smegmatis and the rrnA(s) operon of M. tuberculosis are homologous. The presence of P2 in M. smegmatis and its absence from M. tuberculosis is attributable to insertions/deletions of 97 bp.

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